Loyola A M, Cardoso S V, Lisa G S, Oliveira L J, Mesquita R A, Carmo M A V, Aguiar M C F
Oral Pathology Laboratory, School of Dentistry, Federal University of Uberlândia, Brazil.
Int Endod J. 2005 Jul;38(7):465-9. doi: 10.1111/j.1365-2591.2005.00971.x.
To investigate the occurrence of apoptotic cell death in the epithelium of radicular cysts and to compare its frequency in lesions presenting a distinct functional state.
Twenty radicular cysts were selected and arranged into two groups with 10 lesions in each group: atrophic (quiescent) and hyperplastic (active) epithelium. Morphologic investigations of apoptosis were conducted by means of optic microscopy in haematoxylin and eosin slides. Immunohistochemical techniques to detect the bcl-2 protein were carried out by streptavidin-biotin-peroxidase assay. In both instances, 30 sequential high-power microscopic fields were observed to determine apoptotic (AI) and bcl-2 immunostaining (bcl-2I) indexes. The presence of AI and bcl-2I within the two groups was compared using the t-test. Correlation between the AI and the bcl-2I was investigated using the Spearman test.
Apoptosis was detected in the epithelium of all cysts. Higher AI levels were found in lesions with an atrophic (0.17 +/- 0.19) rather than a hyperplastic (0.10 +/- 0.10) epithelium. The same was found for the bcl-2I levels (0.06 +/- 0.03 vs. 0.04 +/- 0.01, respectively). However, these differences were not statistically significant. A positive and significant correlation was found between AI and bcl-2I.
Apoptosis was always present in the epithelium of the lesions and was more frequent in lesions with atrophic (quiescent) epithelium.
研究根尖囊肿上皮细胞凋亡性细胞死亡的发生情况，并比较其在呈现不同功能状态病变中的发生频率。
选取20个根尖囊肿，分为两组，每组10个病变：萎缩性（静止性）上皮和增生性（活动性）上皮。通过苏木精和伊红染色切片的光学显微镜对凋亡进行形态学研究。采用链霉亲和素-生物素-过氧化物酶法进行免疫组织化学技术检测bcl-2蛋白。在这两种情况下，观察30个连续的高倍显微镜视野以确定凋亡（AI）和bcl-2免疫染色（bcl-2I）指数。使用t检验比较两组中AI和bcl-2I的存在情况。使用Spearman检验研究AI和bcl-2I之间的相关性。
在所有囊肿的上皮中均检测到凋亡。在萎缩性上皮（0.17±0.19）的病变中发现的AI水平高于增生性上皮（0.10±0.10）的病变。bcl-2I水平也是如此（分别为0.06±0.03和0.04±0.01）。然而，这些差异无统计学意义。发现AI和bcl-2I之间存在正相关且具有统计学意义。
凋亡始终存在于病变的上皮中，且在萎缩性（静止性）上皮的病变中更为常见。
