单囊性成釉细胞瘤、牙源性角化囊肿、含牙囊肿及根尖囊肿的增殖和抗凋亡指数。

Proliferative and anti-apoptotic indices of unicystic ameloblastoma, odontogenic keratocyst, dentigerous cyst and radicular cyst.

作者信息

Orikpete Efetobo Victor, Omoregie Osawe Felix, Ojo Michael Akin

机构信息

Department of Oral Pathology and Oral Biology, Faculty of Dentistry, University of Port Harcourt, Port Harcourt, Rivers State, Nigeria.

Department of Oral and Maxillofacial Pathology and Medicine, School of Dentistry, University of Benin, Benin City, Edo State, Nigeria.

出版信息

J Oral Maxillofac Pathol. 2020 May-Aug;24(2):399. doi: 10.4103/jomfp.JOMFP_289_19. Epub 2020 Sep 9.

DOI:10.4103/jomfp.JOMFP_289_19

PMID:33456257

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7802867/

Abstract

OBJECTIVE

The aim of this study is to compare the proliferative capacity and antiapoptotic capacity of unicystic ameloblastoma (UA), odontogenic keratocyst (OKC), dentigerous cyst (DC) and radicular cyst (RC) by assessing the Ki-67 labeling index (LI) and Bcl-2 LI, respectively.

MATERIALS AND METHODS

Formalin-fixed, paraffin-embedded tissue blocks of twenty-three histopathologically diagnosed UAs, 6 OKCs, 8 DCs and 10 RCs were selected from the archival specimens. Five micrometer thick sections of tissue blocks of the lesions were processed for immunohistochemical staining with Ki-67 and Bcl-2. The Ki-67 LI and Bcl-2 LI were determined for the cases with a positive reaction.

RESULTS

Ki-67 expression was seen in 6 (26.1%) cases of UA, 4 (66.7%) OKC, 1 (12.5%) DC and 1 (10.0%) RC. There was a statistically significant difference between the mean Ki-67 LI of UA and OKC ( = 0.024). Bcl-2 expression was seen in 16 (69.6%) UA, 5 (83.3%) OKC, 5 (62.5%) DC and 5 (50.0%) RC. The mean Bcl-2 LI of UA was significantly higher than that of DC ( = 0.048). Furthermore, cases of OKC had significantly higher mean Bcl-2 LI compared to DC ( = 0.026) and RC ( = 0.049).

CONCLUSION

This study suggests that the Ki-67 LI may help in differentiating OKC from UA. The Bcl-2 LI may be useful in differentiating UA from DCs, and differentiating OKC from DC and RC.

摘要

目的

本研究旨在通过分别评估Ki-67标记指数（LI）和Bcl-2 LI，比较单囊性成釉细胞瘤（UA）、牙源性角化囊肿（OKC）、含牙囊肿（DC）和根端囊肿（RC）的增殖能力和抗凋亡能力。

材料与方法

从存档标本中选取23例经组织病理学诊断的UA、6例OKC、8例DC和10例RC的福尔马林固定、石蜡包埋组织块。对病变组织块进行5微米厚切片，用Ki-67和Bcl-2进行免疫组织化学染色。对呈阳性反应的病例测定Ki-67 LI和Bcl-2 LI。

结果

Ki-67表达见于6例（26.1%）UA、4例（66.7%）OKC、1例（12.5%）DC和1例（10.0%）RC。UA和OKC的平均Ki-67 LI之间存在统计学显著差异（P = 0.024）。Bcl-2表达见于16例（69.6%）UA、5例（83.3%）OKC、5例（62.5%）DC和5例（50.0%）RC。UA的平均Bcl-2 LI显著高于DC（P = 0.048）。此外，OKC病例的平均Bcl-2 LI显著高于DC（P = 0.026）和RC（P = 0.049）。