Ojeda-Robertos N F, Mendoza-de Gives P, Torres-Acosta J F J, Rodríguez-Vivas R I, Aguilar-Caballero A J
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Km 15.5 carretera Mérida-Xmatkuil, Mérida, Yucatán, México.
J Helminthol. 2005 Jun;79(2):151-7. doi: 10.1079/joh2005283.
The use of Duddingtonia flagrans in the control of goat nematodes was investigated. Initially, the time of passage of chlamydospores through the digestive tract of goats was evaluated. Two groups of seven parasite-free kids were formed. Group A received a single dose of 3.5x10(6) D. flagrans chlamydospores (FTHO-8 strain) per kg of live weight. Group B did not receive any chlamydospores. Faeces were obtained from each kid daily from day 4 prior to inoculation until day 5 post-inoculation (PI) and were placed in Petri dishes containing water agar. Gastrointestinal nematode infective larvae were added to each Petri dish and incubated at 25 degrees C for 7 days. Petri dishes were examined to detect the fungus and trapped nematodes. A second trial evaluated the effect of D. flagrans on the number of gastrointestinal nematode larvae harvested from goat faecal cultures in naturally infected goats. Two groups of seven goats were formed. The treated group received a single dose of 3.5x10(6) D. flagrans chlamydospores per kg of liveweight. The control group did not receive any chlamydospores. Faeces were obtained twice daily from each kid. Two faecal cultures were made for each kid. One was incubated for 7 days and the other for 14 days. Gastrointestinal nematode larvae were recovered from each culture and counted. Percentage of larval development reduction was determined using a ratio of larvae/eggs deposited in the control and treated groups. Duddingtonia flagrans survived the digestive process of goats, and maintained its predatory activity, being observed from 21 to 81 h PI (3 to 4 days). A reduction in the infective larvae population in the treated group compared to the non-treated group was observed in both incubation periods (7 days: 5.3-36.0%; 14 days: 0-52.8%, P>0.05). Although a single inoculation of D. flagrans can induce a reduction of infective larvae collected from faeces, a different scheme of dosing may be needed to enhance the efficacy of D. flagrans in goats.
研究了杜氏节丛孢菌在控制山羊线虫方面的应用。首先，评估了厚垣孢子通过山羊消化道的时间。将两组无寄生虫的羔羊各分为七只。A组每千克活重单次接种3.5×10(6)个杜氏节丛孢菌厚垣孢子（FTHO - 8菌株）。B组未接种任何厚垣孢子。从接种前第4天到接种后第5天，每天从每只羔羊采集粪便，并置于含有水琼脂的培养皿中。向每个培养皿中添加胃肠道线虫感染性幼虫，并在25℃下孵育7天。检查培养皿以检测真菌和捕获的线虫。第二项试验评估了杜氏节丛孢菌对自然感染山羊粪便培养物中收获的胃肠道线虫幼虫数量的影响。将两组山羊各分为七只。治疗组每千克活重单次接种3.5×10(6)个杜氏节丛孢菌厚垣孢子。对照组未接种任何厚垣孢子。每天从每只羔羊采集两次粪便。为每只羔羊制作两份粪便培养物。一份孵育7天，另一份孵育14天。从每个培养物中回收并计数胃肠道线虫幼虫。使用对照组和治疗组中幼虫/虫卵沉积比例确定幼虫发育减少的百分比。杜氏节丛孢菌在山羊的消化过程中存活下来，并保持其捕食活性，在接种后21至81小时（3至4天）观察到。在两个孵育期（7天：5.3 - 36.0%；14天：0 - 52.8%，P>0.05）中，观察到治疗组与未治疗组相比感染性幼虫数量减少。尽管单次接种杜氏节丛孢菌可使从粪便中收集的感染性幼虫减少，但可能需要不同的给药方案来提高杜氏节丛孢菌对山羊的疗效。
