Ojeda-Robertos N F, Torres-Acosta J F J, Ayala-Burgos A, Aguilar-Caballero A J, Cob-Galera L A, Mendoza-de-Gives P
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Km. 15.5 carretera Mérida-Xmatkuil, Mérida, Yucatán, Mexico.
Vet Parasitol. 2008 Apr 15;152(3-4):339-43. doi: 10.1016/j.vetpar.2007.12.023. Epub 2007 Dec 27.
Previous observations showed that Duddingtonia flagrans chlamydospores were visualized in McMaster chambers containing faeces of treated sheep. This trial explored the McMaster technique as a tool to quantify chlamydospores in sheep faeces. A range of individual chlamydospore doses (from 19.5 x 10(6) to 177.5 x 10(6)) were offered orally to nine lambs for 7 consecutive days. A faecal sample (5 g) was daily obtained from the rectum of each animal (from days 1 to 13) to perform the McMaster technique using a sugar flotation fluid with 1.27 g/mL density. Each chlamydospore counted in the McMaster chamber was considered as 50 chlamydospores per g of faeces (CPG). The results confirmed that the estimated CPG was associated with the daily dose offered to the animals (r(2)=0.90; P<0.001). Furthermore, the total chlamydospore dose received by each animal was strongly associated to the total quantity of CPG obtained from the bulk faeces (TCtot) (r(2)=0.96; P<0.0001). Quantification of CPG can be used as a helpful tool to determine the number of chlamydospores reaching the faeces in orally dosed animals. This could be used to evaluate the efficacy of D. flagrans for the control of gastrointestinal nematode larvae in sheep faeces.
先前的观察表明，在装有经处理绵羊粪便的麦克马斯特计数板中可以看到弗氏内孢霉厚垣孢子。本试验探索了将麦克马斯特技术作为一种量化绵羊粪便中厚垣孢子的工具。给9只羔羊连续7天口服一系列不同剂量的单个厚垣孢子（从19.5×10⁶到177.5×10⁶）。每天（从第1天到第13天）从每只动物的直肠采集5克粪便样本，使用密度为1.27克/毫升的糖浮选液进行麦克马斯特技术检测。在麦克马斯特计数板中计数的每个厚垣孢子相当于每克粪便中有50个厚垣孢子（CPG）。结果证实，估计的CPG与给动物投喂的每日剂量相关（r² = 0.90；P < 0.001）。此外，每只动物接受的厚垣孢子总剂量与从大量粪便中获得的CPG总量（TCtot）密切相关（r² = 0.96；P < 0.0001）。CPG的量化可作为一种有用的工具，用于确定经口服给药的动物粪便中厚垣孢子的数量。这可用于评估弗氏内孢霉对控制绵羊粪便中胃肠道线虫幼虫的效果。
