Lin Qiu-Ye, Jin Li-Ji, Ma Yong-Sheng, Shi Ming, Xu Yong-Ping
Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian, People's Republic of China.
Phytother Res. 2007 Sep;21(9):879-83. doi: 10.1002/ptr.2171.
Excess nitric oxide (NO) production has been implicated in inflammatory diseases. The present study investigated the inhibitory effect of the stem bark extract of Acanthopanax senticosus (A. senticosus) on NO production in murine macrophages in vitro and in vivo. In vitro exposure of RAW264.7 cells to 1, 10, 50, 100, 250, 500 and 1000 microg/mL of A. senticosus extract significantly suppressed NO production induced by lipopolysaccharide (LPS) and interferon gamma (IFN-gamma) in a dose-dependent manner. In vitro exposure of mouse resident peritoneal macrophages to 1, 10, 100 and 1000 microg/mL of A. senticosus extract significantly suppressed NO production induced by LPS and IFN-gamma in a dose-dependent manner. In vivo administration of A. senticosus extract (50, 100 and 200 mg/kg) to KM mice dose-dependently inhibited LPS and IFN-gamma induced production of NO in isolated mouse peritoneal macrophages ex vivo. Exposure to A. senticosus extract had no effect on cell viability and systemic toxicity. The results demonstrated that the stem bark extract of A. senticosus extract inhibits NO production in murine macrophages in vitro and in vivo.
过量一氧化氮（NO）的产生与炎症性疾病有关。本研究调查了刺五加茎皮提取物对体外和体内小鼠巨噬细胞中NO产生的抑制作用。将RAW264.7细胞体外暴露于1、10、50、100、250、500和1000μg/mL的刺五加提取物中，可显著抑制脂多糖（LPS）和干扰素γ（IFN-γ）诱导的NO产生，且呈剂量依赖性。将小鼠常驻腹膜巨噬细胞体外暴露于1、10、100和1000μg/mL的刺五加提取物中，可显著抑制LPS和IFN-γ诱导的NO产生，且呈剂量依赖性。对KM小鼠体内给予刺五加提取物（50、100和200mg/kg），可剂量依赖性地抑制离体小鼠腹膜巨噬细胞中LPS和IFN-γ诱导的NO产生。暴露于刺五加提取物对细胞活力和全身毒性无影响。结果表明，刺五加茎皮提取物在体外和体内均可抑制小鼠巨噬细胞中NO的产生。
