Rozynek Elzbieta, Dzierzanowska-Fangrat Katarzyna, Jozwiak Paulina, Popowski Janusz, Korsak Dorota, Dzierzanowska Danuta
Department of Clinical Microbiology and Immunology, Children's Memorial Health Institute, 04-730 Warsaw, Aleja Dzieci Polskich 20, Poland 2Department of Safety of Food and Nutrition, National Food and Nutrition Institute, 02-903 Warsaw, Powsinska 61/63, Poland.
J Med Microbiol. 2005 Jul;54(Pt 7):615-619. doi: 10.1099/jmm.0.45988-0.
The pathogenicity of thermotolerant Campylobacter species, common food-borne pathogens, depends on certain factors unevenly distributed among strains of different origin. The prevalence of such markers has never been examined in a population of Polish Campylobacter strains of human and poultry origin. Therefore, we analysed the presence of the cadF, cdtA, cdtB and cdtC genes and the iam sequence in Campylobacter jejuni (n = 115) and Campylobacter coli (n = 57) isolates from children with diarrhoea and from chicken carcasses. The cadF gene was present in nearly 100% of Campylobacter isolates tested, regardless of their origin or species. In contrast, the iam region was found in 83.3% and 100% of C. coli isolates from children and chickens, respectively, but in only 1.6% and 54.7%, respectively, of C. jejuni isolates. Similarly, the detection rates of cdt genes varied between human and chicken isolates. All three cdt genes were found in nearly all C. jejuni isolates from both children and chickens, but in only 5.6% of human C. coli isolates as compared to 87.2% of chicken C. coli isolates. This different distribution of genetic markers between human and chicken isolates indicates that some Campylobacter infections in children may have additional sources other than contaminated chicken meat.
耐热弯曲杆菌属是常见的食源性病原体，其致病性取决于某些在不同来源菌株中分布不均的因素。此类标志物在波兰人类和家禽源弯曲杆菌菌株群体中的流行情况从未得到过研究。因此，我们分析了来自腹泻儿童和鸡 carcasses 的空肠弯曲杆菌（n = 115）和结肠弯曲杆菌（n = 57）分离株中 cadF、cdtA、cdtB 和 cdtC 基因以及 iam 序列的存在情况。无论其来源或种类如何，cadF 基因存在于近 100% 的测试弯曲杆菌分离株中。相比之下，iam 区域分别在 83.3% 的儿童源和 100% 的鸡源结肠弯曲杆菌分离株中被发现，但在空肠弯曲杆菌分离株中分别仅为 1.6% 和 54.7%。同样，cdt 基因的检出率在人类和鸡的分离株之间也有所不同。在几乎所有来自儿童和鸡的空肠弯曲杆菌分离株中都发现了所有三个 cdt 基因，但在人类结肠弯曲杆菌分离株中仅为 5.6%，而在鸡源结肠弯曲杆菌分离株中为 87.2%。人类和鸡分离株之间遗传标志物的这种不同分布表明，儿童中的一些弯曲杆菌感染可能有除受污染鸡肉之外的其他来源。 （注：carcasses 原意为“屠体、畜体”，这里结合语境可理解为鸡的躯体部分，翻译为“鸡 carcasses”更便于理解，但在正式文本中可根据具体情况更准确表述，比如“鸡胴体”等）
