从肉鸡胴体和肉鸡屠宰场分离的空肠弯曲菌中致病性基因的鉴定。
Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses.
机构信息
Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Goncalves 8824, Porto Alegre, Rio Grande do Sul, CEP 91540-000, Brazil.
Faculdade de Veterinária, Universidade de Passo Fundo, Passo Fundo, Brazil.
出版信息
Sci Rep. 2021 Feb 25;11(1):4588. doi: 10.1038/s41598-021-84149-1.
Campylobacter jejuni is one of the most common causes of foodborne diseases worldwide. There are few reports on Campylobacter strains isolated from Latin-American countries. Here, 140 C. jejuni strains isolated from cloacal and transport boxes swabs, water from chiller tanks, and broiler carcasses of five poultry companies in Southern Brazil were identified using phenotypic and genotypic methods. Polymerase chain reaction (PCR) was used to analyze eight C. jejuni virulence markers: flaA, cadF, and invasion-associated (iam) genes, cdtABC operon (associated with the cytolethal distending toxin), and plasmidial virB11 and wlaN genes were present in 78.5%, 77.8%, 0%, 74.2%, 22.1%, and 10.7% of samples, respectively. There were 25 different virulence profiles: 1 (cdtA, cdtB, cdtC, flaA, and cadF), 2 (cdtA, cdtB, cdtC, flaA, cadF, and virB11), and 3 (cdtA, cdtB, cdtC, flaA, cadF, and wlaN) were the most common (> 60% of strains). We provide insight into factors related to the occurrence of this pathogen and their epidemiology.
空肠弯曲菌是世界范围内最常见的食源性病原体之一。有关拉丁美洲国家分离的空肠弯曲菌菌株的报道较少。本研究采用表型和基因型方法对来自巴西南部 5 家家禽公司的直肠和运输箱拭子、冷水机组水箱水和肉鸡胴体中的 140 株空肠弯曲菌进行了鉴定。聚合酶链反应（PCR）用于分析 8 种空肠弯曲菌毒力标记物：flaA、cadF 和侵袭相关（iam）基因、cdtABC 操纵子（与细胞致死扩张毒素相关）和质粒 virB11 和 wlaN 基因，分别在 78.5%、77.8%、0%、74.2%、22.1%和 10.7%的样本中存在。有 25 种不同的毒力谱：1（cdtA、cdtB、cdtC、flaA 和 cadF）、2（cdtA、cdtB、cdtC、flaA、cadF 和 virB11）和 3（cdtA、cdtB、cdtC、flaA、cadF 和 wlaN）最为常见（>60%的菌株）。本研究深入了解了与该病原体发生及其流行病学相关的因素。
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