de Oliveira Mauricéia Greici, Rizzi Caroline, Galli Vanessa, Lopes Graciela Volz, Haubert Louise, Dellagostin Odir Antônio, da Silva Wladimir Padilha
a Departamento de Ciência e Tecnologia Agroindustrial, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
b Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Can J Microbiol. 2019 Apr;65(4):253-260. doi: 10.1139/cjm-2018-0539. Epub 2018 Dec 11.
The aims of this study were to evaluate the presence of genes associated with adhesion (cadF), invasion (ciaB), and cytotoxin production (cdtA, cdtB, and cdtC) among Campylobacter jejuni isolates from a poultry slaughterhouse and to investigate the effect of different temperatures on the expression of these virulence-associated genes. A total of 88 C. jejuni isolates from cecum, liver, chicken carcasses, chilled water, and scalding water were submitted to PCR assay for detection of virulence genes. Representative isolates were selected for gene expression evaluation at 37 and 42 °C, according to their virulence gene profile and genotypic typing. All C. jejuni isolates carried the five virulence-associated genes, which play an important role in the infectious process. Differential gene expression by RT-qPCR was observed among C. jejuni isolates at 37 and 42 °C. The expression levels at 37 °C showed upregulation of the ciaB, cdtA, cdtB, and cdtC genes in five isolates, with the exception of ciaB for isolate 4. At 42 °C, upregulation was observed for ciaB and cdtC, cdtA and cdtB, and cadF in four, three, and two isolates, respectively. The C. jejuni isolates expressed the virulence genes evaluated, and the expression is gene- and isolate-dependent and varied according the temperature.
本研究的目的是评估来自家禽屠宰场的空肠弯曲菌分离株中与黏附相关基因（cadF）、侵袭相关基因（ciaB）和细胞毒素产生相关基因（cdtA、cdtB和cdtC）的存在情况，并研究不同温度对这些毒力相关基因表达的影响。总共88株来自盲肠、肝脏、鸡胴体、冷水和烫毛水的空肠弯曲菌分离株接受了用于检测毒力基因的PCR检测。根据其毒力基因谱和基因分型，选择代表性分离株在37℃和42℃下进行基因表达评估。所有空肠弯曲菌分离株均携带这五个毒力相关基因，它们在感染过程中起重要作用。在37℃和42℃下的空肠弯曲菌分离株中观察到了通过RT-qPCR检测的差异基因表达。在37℃时，五个分离株中ciaB、cdtA、cdtB和cdtC基因表达上调，但分离株4的ciaB基因除外。在42℃时，分别在四个、三个和两个分离株中观察到ciaB和cdtC、cdtA和cdtB以及cadF基因表达上调。空肠弯曲菌分离株表达了所评估的毒力基因，且其表达具有基因和分离株依赖性，并随温度而变化。
