在胶原凝胶中培养的成年胰岛会转分化为导管样细胞。
Adult islets cultured in collagen gel transdifferentiate into duct-like cells.
作者信息
Lu Jin, Gu Ya-Peng, Xu Xia, Liu Mei-Lian, Xie Ping, Song Hui-Ping
机构信息
Department of Biochemistry, Xiangya School of Medicine, Central South University, Xiangya Road, Changsha 410078 Hunan Province, China.
出版信息
World J Gastroenterol. 2005 Jun 14;11(22):3426-30. doi: 10.3748/wjg.v11.i22.3426.
AIM
To establish a model of islet-ductal cell transdifferentiation to identify the transdifferentiated cells.
METHODS
Collagen was extracted from rat tail at first. Purified rat islets were divided into three groups, embedded in collagen gel and incubated respectively in DMEM/F12 alone (control group), DMEM/F12 plus epidermal growth factor (EGF), DMEM/F12 plus EGF and cholera toxin (CT). Transdifferentiation was proved by microscopy, RT-PCR, immunohistochemistry and RIA.
RESULTS
Islets embedded in collagen gel plus EGF and CT were cystically transformed and could express new gene cytokeratin 19 while still maintaining the expression of insulin and Pdx-1 genes. Immunohistochemistry demonstrated that the protein of cytokeratin 19 was only expressed in the third group. The insulin content secreted by islets in the third group decreased significantly during the transdifferentiation.
CONCLUSION
CT is a crucial factor for the islet-ductal cell transdifferentiation.
目的
建立胰岛-导管细胞转分化模型以鉴定转分化细胞。
方法
首先从大鼠尾部提取胶原蛋白。将纯化的大鼠胰岛分为三组，包埋于胶原蛋白凝胶中，分别在单纯DMEM/F12（对照组）、DMEM/F12加表皮生长因子（EGF）、DMEM/F12加EGF和霍乱毒素（CT）中孵育。通过显微镜检查、逆转录-聚合酶链反应（RT-PCR）、免疫组织化学和放射免疫分析（RIA）证实转分化。
结果
包埋于胶原蛋白凝胶加EGF和CT中的胰岛发生囊性转化，可表达新基因细胞角蛋白19，同时仍维持胰岛素和胰腺十二指肠同源盒基因-1（Pdx-1）的表达。免疫组织化学显示细胞角蛋白19蛋白仅在第三组中表达。在转分化过程中，第三组胰岛分泌的胰岛素含量显著降低。
结论
CT是胰岛-导管细胞转分化的关键因素。
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