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本文引用的文献

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Transdifferentiation of blood-derived human adult endothelial progenitor cells into functionally active cardiomyocytes.血液来源的人类成人内皮祖细胞向功能活跃的心肌细胞的转分化。
Circulation. 2003 Feb 25;107(7):1024-32. doi: 10.1161/01.cir.0000051460.85800.bb.
2
Factors mediating the transdifferentiation of islets of Langerhans to duct epithelial-like structures.介导胰岛向导管上皮样结构转分化的因素。
J Endocrinol. 2001 Nov;171(2):309-18. doi: 10.1677/joe.0.1710309.
3
Transdifferentiation of human islet cells in a long-term culture.人胰岛细胞在长期培养中的转分化
Pancreas. 2001 Aug;23(2):157-71. doi: 10.1097/00006676-200108000-00006.
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In vitro cultivation of human islets from expanded ductal tissue.从扩增的导管组织中体外培养人胰岛。
Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7999-8004. doi: 10.1073/pnas.97.14.7999.
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Tubular epithelial-myofibroblast transdifferentiation in progressive tubulointerstitial fibrosis in 5/6 nephrectomized rats.5/6肾切除大鼠进行性肾小管间质纤维化中的肾小管上皮-肌成纤维细胞转分化
Kidney Int. 1998 Sep;54(3):864-76. doi: 10.1046/j.1523-1755.1998.00076.x.
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Transdifferentiation of human islets to pancreatic ductal cells in collagen matrix culture.在胶原基质培养中人类胰岛向胰腺导管细胞的转分化
Differentiation. 1996 Oct;61(1):67-75. doi: 10.1046/j.1432-0436.1996.6110067.x.
7
Regulation of fibroblast proliferation in three-dimensional collagen gel matrix.三维胶原凝胶基质中 成纤维细胞增殖的调控
In Vitro Cell Dev Biol Anim. 1996 Jul-Aug;32(7):427-33. doi: 10.1007/BF02723005.
8
Differentiation of pancreatic acinar cells into duct-like cells in vitro.体外胰腺腺泡细胞向导管样细胞的分化
Lab Invest. 1993 Nov;69(5):518-30.
9
A second pathway for regeneration of adult exocrine and endocrine pancreas. A possible recapitulation of embryonic development.成年外分泌和内分泌胰腺再生的第二条途径。胚胎发育的一种可能重演。
Diabetes. 1993 Dec;42(12):1715-20. doi: 10.2337/diab.42.12.1715.
10
The epidermal growth factor.表皮生长因子。
Cell Biol Int. 1995 May;19(5):413-30. doi: 10.1006/cbir.1995.1086.

在胶原凝胶中培养的成年胰岛会转分化为导管样细胞。

Adult islets cultured in collagen gel transdifferentiate into duct-like cells.

作者信息

Lu Jin, Gu Ya-Peng, Xu Xia, Liu Mei-Lian, Xie Ping, Song Hui-Ping

机构信息

Department of Biochemistry, Xiangya School of Medicine, Central South University, Xiangya Road, Changsha 410078 Hunan Province, China.

出版信息

World J Gastroenterol. 2005 Jun 14;11(22):3426-30. doi: 10.3748/wjg.v11.i22.3426.

DOI:10.3748/wjg.v11.i22.3426
PMID:15948249
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4315998/
Abstract

AIM

To establish a model of islet-ductal cell transdifferentiation to identify the transdifferentiated cells.

METHODS

Collagen was extracted from rat tail at first. Purified rat islets were divided into three groups, embedded in collagen gel and incubated respectively in DMEM/F12 alone (control group), DMEM/F12 plus epidermal growth factor (EGF), DMEM/F12 plus EGF and cholera toxin (CT). Transdifferentiation was proved by microscopy, RT-PCR, immunohistochemistry and RIA.

RESULTS

Islets embedded in collagen gel plus EGF and CT were cystically transformed and could express new gene cytokeratin 19 while still maintaining the expression of insulin and Pdx-1 genes. Immunohistochemistry demonstrated that the protein of cytokeratin 19 was only expressed in the third group. The insulin content secreted by islets in the third group decreased significantly during the transdifferentiation.

CONCLUSION

CT is a crucial factor for the islet-ductal cell transdifferentiation.

摘要

目的

建立胰岛-导管细胞转分化模型以鉴定转分化细胞。

方法

首先从大鼠尾部提取胶原蛋白。将纯化的大鼠胰岛分为三组，包埋于胶原蛋白凝胶中，分别在单纯DMEM/F12（对照组）、DMEM/F12加表皮生长因子（EGF）、DMEM/F12加EGF和霍乱毒素（CT）中孵育。通过显微镜检查、逆转录-聚合酶链反应（RT-PCR）、免疫组织化学和放射免疫分析（RIA）证实转分化。

结果

包埋于胶原蛋白凝胶加EGF和CT中的胰岛发生囊性转化，可表达新基因细胞角蛋白19，同时仍维持胰岛素和胰腺十二指肠同源盒基因-1（Pdx-1）的表达。免疫组织化学显示细胞角蛋白19蛋白仅在第三组中表达。在转分化过程中，第三组胰岛分泌的胰岛素含量显著降低。

结论

CT是胰岛-导管细胞转分化的关键因素。