Figueredo C M S, Fischer R G, Gustafsson A
Division of Periodontology, Institute of Odontology, Karolinska Institutet, Stockholm, Sweden.
J Periodontol. 2005 Jun;76(6):951-5. doi: 10.1902/jop.2005.76.6.951.
The aim of this study was to compare the activity of neutrophilic granulocytes in patients with severe periodontitis and patients with gingivitis alone.
The study population comprised 22 patients with gingivitis and 44 with periodontitis. Samples of gingival crevicular fluid (GCF) were collected from untreated patients with gingivitis and from shallow and deep pockets in untreated patients with periodontitis. GCF samples were analyzed for lactoferrin, elastase, matrix metalloproteinase-8 and -9, and collagenolytic activity.
The free elastase activity and the neutrophil activity, estimated as the ratio between elastase and lactoferrin, were significantly higher in the samples from the periodontitis patients. These differences were also observed in shallow pockets in periodontitis patients compared to similar pockets in patients with gingivitis.
This study shows higher levels of free elastase in untreated patients with periodontitis, relative to inflammation-matched controls, which may explain the tissue destruction seen in periodontitis.
本研究旨在比较重度牙周炎患者和单纯牙龈炎患者中性粒细胞的活性。
研究对象包括22例牙龈炎患者和44例牙周炎患者。从未经治疗的牙龈炎患者以及未经治疗的牙周炎患者的浅袋和深袋中采集龈沟液（GCF）样本。对GCF样本进行乳铁蛋白、弹性蛋白酶、基质金属蛋白酶-8和-9以及胶原olytic活性分析。
牙周炎患者样本中的游离弹性蛋白酶活性和以弹性蛋白酶与乳铁蛋白之比估算的中性粒细胞活性显著更高。与牙龈炎患者的类似牙周袋相比，在牙周炎患者的浅袋中也观察到了这些差异。
本研究表明，未经治疗的牙周炎患者的游离弹性蛋白酶水平相对于炎症匹配的对照组更高，这可能解释了牙周炎中所见的组织破坏。
