Armstead I P, Skøt L, Turner L B, Skøt K, Donnison I S, Humphreys M O, King I P
Plant Genetics and Breeding Department, Institute of Grassland and Environmental Research, Aberystwyth, Ceredigion SY23 3EB, UK.
New Phytol. 2005 Jul;167(1):239-47. doi: 10.1111/j.1469-8137.2005.01392.x.
Microsynteny with rice and comparative genetic mapping were used to identify candidate orthologous sequences to the rice Hd1(Se1) gene in Lolium perenne and Festuca pratensis. A F. pratensis bacterial artificial chromosome (BAC) library was screened with a marker (S2539) physically close to Hd1 in rice to identify the equivalent genomic region in F. pratensis. The BAC sequence was used to identify and map the same region in L. perenne. Predicted protein sequences for L. perenne and F. pratensis Hd1 candidates (LpHd1 and FpHd1) indicated they were CONSTANS-like zinc finger proteins with 61-62% sequence identity with rice Hd1 and 72% identity with barley HvCO1. LpHd1 and FpHd1 were physically linked in their respective genomes (< 4 kb) to marker S2539, which was mapped to L. perenne chromosome 7. The identified candidate orthologues of rice Hd1 and barley HvCO1 in L. perenne and F. pratensis map to chromosome 7, a region of the L. perenne genome which has a degree of conserved genetic synteny both with rice chromosome 6, which contains Hd1, and barley chromosome 7H, which contains HvCO1.
利用与水稻的微同线性和比较遗传图谱，在多年生黑麦草和草地羊茅中鉴定与水稻Hd1(Se1)基因的候选直系同源序列。用一个在物理位置上与水稻Hd1基因相近的标记（S2539）筛选草地羊茅细菌人工染色体（BAC）文库，以鉴定草地羊茅中的等效基因组区域。该BAC序列用于鉴定和定位多年生黑麦草中的相同区域。多年生黑麦草和草地羊茅Hd1候选基因（LpHd1和FpHd1）的预测蛋白质序列表明，它们是CONSTANS样锌指蛋白，与水稻Hd1的序列同一性为61%-62%，与大麦HvCO1的同一性为72%。LpHd1和FpHd1在各自基因组中与标记S2539物理连锁（<4 kb），该标记被定位到多年生黑麦草的7号染色体上。在多年生黑麦草和草地羊茅中鉴定出的水稻Hd1和大麦HvCO1的候选直系同源基因定位于7号染色体，这是多年生黑麦草基因组中的一个区域，该区域与含有Hd1的水稻6号染色体以及含有HvCO1的大麦7H染色体具有一定程度的保守遗传同线性。
