Yang Bicheng, Thorogood Daniel, Armstead Ian P, Franklin F C H, Barth Susanne
Teagasc Crops Research Centre, Oak Park, Carlow, Ireland.
Plant Mol Biol. 2009 Aug;70(6):709-23. doi: 10.1007/s11103-009-9501-2. Epub 2009 May 30.
Self-incompatibility (SI) in Lolium perenne is controlled gametophytically by the S-Z two-locus system. S and Z loci mapped to L. perenne linkage groups 1 and 2, respectively, with their corresponding putative-syntenic regions on rice chromosome 5 (R5) and R4. None of the gene products of S and Z have yet been identified. SI cDNA libraries were developed to enrich for SI expressed genes in L. perenne. Transcripts were identified from the SI libraries that were orthologous to sequences on rice R4 and R5. These represent potential SI candidate genes. Altogether ten expressed SI candidate genes were identified. A rapid increase in gene expression within two minutes after pollen-stigma contact was revealed, reaching a maximum between 2 and 10 min. The potential involvement of these genes in the SI reactions is discussed.
多年生黑麦草的自交不亲和性（SI）由S-Z双位点系统配子体控制。S和Z位点分别定位于多年生黑麦草的连锁群1和2上，它们在水稻第5号染色体（R5）和第4号染色体（R4）上有相应的假定同线区域。S和Z的基因产物尚未被鉴定出来。构建了SI cDNA文库以富集多年生黑麦草中SI表达基因。从SI文库中鉴定出与水稻R4和R5上的序列直系同源的转录本。这些代表潜在的SI候选基因。总共鉴定出10个表达的SI候选基因。结果表明，花粉与柱头接触后两分钟内基因表达迅速增加，在2至10分钟之间达到最大值。讨论了这些基因在SI反应中的潜在作用。
