Williamson Matthew Richard, Adams Eric F, Coombes Allan G A
Aston Pharmacy School, Aston University, Aston Triangle, Birmingham, B4 7ET, UK.
Biomaterials. 2006 Mar;27(7):1019-26. doi: 10.1016/j.biomaterials.2005.06.018. Epub 2005 Jul 28.
Poly(epsilon-caprolactone) (PCL) fibres were produced by wet spinning from solutions in acetone under low shear (gravity flow) conditions. As-spun PCL fibres exhibited a mean strength and stiffness of 7.9 MPa and 0.1 GPa, respectively and a rough, porous surface morphology. Cold drawing to an extension of 500% resulted in increases in fibre strength (43 MPa) and stiffness (0.3 GPa) and development of an oriented, fibrillar surface texture. The proliferation rate of Swiss 3T3 mouse fibroblasts and C2C12 mouse myoblasts on as-spun, 500% cold-drawn and gelatin-modified PCL fibres was determined in cell culture to provide a basic measure of the biocompatibility of the fibres. Proliferation of both cell types was consistently higher on gelatin-coated fibres relative to as-spun fibres at time points below 7 days. Fibroblast growth rates on cold-drawn PCL fibres exceeded those on as-spun fibres but myoblast proliferation was similar on both substrates. After 1 day in culture, both cell types had spread and coalesced on the fibres to form a cell layer, which conformed closely to the underlying topography. The high fibre compliance combined with a potential for modifying the fibre surface chemistry with cell adhesion molecules and the surface architecture by cold drawing to enhance proliferation of fibroblasts and myoblasts, recommends further investigation of gravity-spun PCL fibres for 3-D scaffold production in soft tissue engineering.
聚（ε-己内酯）（PCL）纤维是在低剪切（重力流动）条件下，通过从丙酮溶液中湿法纺丝制备的。初纺的PCL纤维平均强度和刚度分别为7.9 MPa和0.1 GPa，表面形态粗糙且多孔。冷拉伸至500%的伸长率导致纤维强度（43 MPa）和刚度（0.3 GPa）增加，并形成了取向的纤维状表面纹理。在细胞培养中测定了瑞士3T3小鼠成纤维细胞和C2C12小鼠成肌细胞在初纺、500%冷拉伸和明胶改性的PCL纤维上的增殖率，以提供纤维生物相容性的基本衡量标准。在7天以下的时间点，相对于初纺纤维，两种细胞类型在明胶包被的纤维上的增殖始终更高。冷拉伸PCL纤维上的成纤维细胞生长速率超过初纺纤维上的，但两种底物上的成肌细胞增殖相似。培养1天后，两种细胞类型都已在纤维上铺展并融合形成细胞层，该细胞层与下面的地形紧密贴合。高纤维顺应性，以及通过细胞粘附分子修饰纤维表面化学性质和通过冷拉伸改变表面结构以增强成纤维细胞和成肌细胞增殖的潜力，建议对重力纺丝PCL纤维在软组织工程中用于三维支架生产进行进一步研究。
