Jacobsen S, Kjelgaard-Hansen M, Hagbard Petersen H, Jensen A L
Department of Large Animal Sciences, The Royal Veterinary and Agricultural University, Dyrlagevej 48, 1870 Frederiksberg C, Copenhagen, Denmark.
Vet J. 2006 Sep;172(2):315-9. doi: 10.1016/j.tvjl.2005.04.021. Epub 2005 Jun 13.
The aim of the present study was to evaluate whether equine serum amyloid A (SAA) concentrations could be measured reliably with a turbidometric immunoassay (TIA) developed for use with human serum. Intra- and inter-assay imprecision were evaluated by multiple measurements on equine serum pools. Assay inaccuracy was determined by linearity under dilution. The assay was subsequently used for measuring SAA concentrations in clinically healthy horses, horses with inflammatory diseases, horses with non-inflammatory diseases, and in horses before and after castration. In pools with low, intermediate and high SAA concentrations, the intra-assay imprecisions were 24.4%, 1.6% and 2.1%, and the inter-assay imprecisions were 33.2%, 4.6% and 6.5%. Slight signs of inaccuracy were observed, but these inaccuracies were negligible when considering the large dynamic range of the SAA response. The assay was able to detect the expected difference in SAA levels in different groups of horses. It was also able to demonstrate the expected dynamic changes in SAA after castration. In conclusion, equine SAA concentrations can be measured reliably using the TIA designed for human SAA.
本研究的目的是评估是否可以使用一种为检测人血清而开发的比浊免疫分析法（TIA）可靠地测定马血清淀粉样蛋白A（SAA）浓度。通过对马血清混合样本进行多次测量来评估批内和批间不精密度。通过稀释线性度来确定分析方法的不准确性。随后该分析方法被用于测量临床健康马匹、患有炎症性疾病的马匹、患有非炎症性疾病的马匹以及去势前后马匹的SAA浓度。在低、中、高SAA浓度的混合样本中，批内不精密度分别为24.4%、1.6%和2.1%，批间不精密度分别为33.2%、4.6%和6.5%。观察到了轻微的不准确迹象，但考虑到SAA反应的较大动态范围，这些不准确可以忽略不计。该分析方法能够检测出不同组马匹中SAA水平的预期差异。它还能够证明去势后SAA的预期动态变化。总之，使用为检测人SAA设计的TIA能够可靠地测量马的SAA浓度。
