Zhang Tianmu, Li Yijun, Chen Chunfei, Liu Xiaoping, Tian Yan, Zeng Siyu, He Miao
Center for Sensor Technology of Environment and Health, Tsinghua University Beijing 100084 China
State Key Joint Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University Beijing 100084 China.
RSC Adv. 2019 Nov 25;9(66):38422-38429. doi: 10.1039/c9ra06796e.
Antibiotics are commonly used in livestock-related agriculture and aquaculture, but they also remain in water and potentially threaten human health. Immunosensors are attractive tools for the rapid detection of antibiotics in water due to their high sensitivity and low costs. However, the simultaneous detection of multi-class antibiotics remains a challenge due to the limited number of detection sites on the immunochip. Also, matrix effects hinder the practical application of these sensors. This paper presents a method for multi-class antibiotic detection in real water using a planar waveguide immunosensor (PWI). We integrate the screening and quantitive detection sites on the same immunochip, and a single screening detection site could detect multi-class antibiotics from the same family, increasing the detection types of analytes. In addition, to eliminate the matrix effects, we develop a testing buffer for real water detection, so that complex pretreatments of the samples can be omitted. Using our sensor and testing buffer, we detect 14 different antibiotics in real water. Lincomycin can be detected with a detection limit of 0.01 μg L, and 13 quinolones can be screened in a single assay. These results demonstrate that this planar waveguide immunosensor is capable of simultaneous screening and quantification of multi-class antibiotic pollutants and is expected to be applied for practical environmental monitoring.
抗生素常用于与家畜相关的农业和水产养殖中，但它们也会残留在水中，并可能威胁人类健康。免疫传感器因其高灵敏度和低成本，是快速检测水中抗生素的有吸引力的工具。然而，由于免疫芯片上检测位点数量有限，同时检测多类抗生素仍然是一个挑战。此外，基质效应阻碍了这些传感器的实际应用。本文提出了一种使用平面波导免疫传感器（PWI）在实际水样中检测多类抗生素的方法。我们在同一免疫芯片上整合了筛选和定量检测位点，单个筛选检测位点可以检测同一类别的多类抗生素，增加了分析物的检测类型。此外，为了消除基质效应，我们开发了一种用于实际水样检测的测试缓冲液，从而可以省略样品的复杂预处理。使用我们的传感器和测试缓冲液，我们在实际水样中检测了14种不同的抗生素。林可霉素的检测限为0.01 μg/L，并且可以在一次检测中筛选出13种喹诺酮类药物。这些结果表明，这种平面波导免疫传感器能够同时筛选和定量多类抗生素污染物，有望应用于实际环境监测。
