Pharmacokinetic Laboratory, Department of Clinical Pharmacy, Center for Clinical Pharmacy and Sciences, School of Pharmacy, Kitasato University, 5-9-1 Shirogane, Minato, Tokyo 108-8641, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 1;878(19):1555-61. doi: 10.1016/j.jchromb.2010.04.012. Epub 2010 May 1.
In this study, three fluoroquinolones, pazufloxacin, ciprofloxacin and levofloxacin, were simultaneously determined in spiked human serum by high-performance liquid chromatography (HPLC) method with fluorescence detection. Chromatography was performed using a C8 column with an isocratic mobile phase consisting of 1% triethylamine (pH 3.0)/acetonitrile (86/14, v/v). Protein precipitation was conducted using perchloric acid and methanol. The calibration curves for the three fluoroquinolones were linear over concentrations ranging from 0.1 to 20.0 microg/mL. The within-day and between-day coefficients of variation obtained from three fluoroquinolones were less than 7%, and relative errors ranged from -1.6% to 9.3%. Mean recoveries of pazufloxacin, ciprofloxacin, and levofloxacin from spiked human serum were 97%, 88%, and 90%, respectively. The proposed method proved to be simple and reliable for the determination of three fluoroquinolones.
本研究采用高效液相色谱-荧光检测法同时测定人血清中三种氟喹诺酮类药物：帕珠沙星、环丙沙星和左氧氟沙星。色谱分析采用 C8 柱，等度洗脱，流动相由 1%三乙胺（pH3.0）/乙腈（86/14，v/v）组成。采用高氯酸-甲醇沉淀蛋白。三种氟喹诺酮类药物的校准曲线在 0.1-20.0μg/mL 浓度范围内呈线性。三种氟喹诺酮类药物的日内和日间变异系数均小于 7%，相对误差在-1.6%至 9.3%之间。帕珠沙星、环丙沙星和左氧氟沙星在人血清中的平均回收率分别为 97%、88%和 90%。该方法简单可靠，可用于三种氟喹诺酮类药物的测定。
