黑麦微卫星间序列（SCIMs）：染色体定位与遗传继承

Secale cereale inter-microsatellites (SCIMs): chromosomal location and genetic inheritance.

作者信息

Camacho M V, Matos M, González C, Pérez-Flores V, Pernaute B, Pinto-Carnide O, Benito C

机构信息

Departamento de Genética, Facultad de Biologia, Universidad Complutense, 28040-Madrid, Spain.

出版信息

Genetica. 2005 Mar;123(3):303-11. doi: 10.1007/s10709-004-5553-z.

DOI:10.1007/s10709-004-5553-z

PMID:15954501

Abstract

The polymerase chain reaction (PCR) was used to locate Sectale cereale (inter-simple sequence repeat ISSR) or Secale cereale inter-microsatellite (SCIM) markers using wheat-rye addition lines in order to develop a set of molecular markers distributed on the seven rye chromosomes. The number of SCIM markers located on 1R, 2R, 3R, 4R, 5R, 6R and 7R chromosomes were 4, 3, 12, 3, 2, 9 and 8, respectively. Therefore, a total of 41 new SCIMs were located on the seven rye chromosomes. The segregation of the 63 different SCIM markers in three different F2 was studied. The observed ISSR segregations were the 3:1 (50.7%), the 15:1 (12.7%) and the 1:1 (14.2%). The linkage analysis carried out indicated that seven of the segregating SCIMs were linked to chromosome 7R and two were linked to chromosome 4R. The use of the SCIM markers as a source of molecular markers that could be linked to interesting genes or other important agronomic traits is discussed.

摘要

为了开发一套分布在七条黑麦染色体上的分子标记，利用小麦-黑麦附加系，通过聚合酶链反应（PCR）来定位黑麦（简单序列重复区间ISSR）或黑麦微卫星间（SCIM）标记。位于1R、2R、3R、4R、5R、6R和7R染色体上的SCIM标记数量分别为4、3、12、3、2、9和8。因此，七条黑麦染色体上共定位到41个新的SCIM标记。研究了63个不同SCIM标记在三个不同F2群体中的分离情况。观察到的ISSR分离比例为3:1（50.7%）、15:1（12.7%）和1:1（14.2%）。进行的连锁分析表明，七个分离的SCIM标记与7R染色体连锁，两个与4R染色体连锁。本文还讨论了将SCIM标记用作可与感兴趣基因或其他重要农艺性状连锁的分子标记来源的用途。