Li Jianjian, Nasuda Shuhei, Endo Takashi R
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University.
Genes Genet Syst. 2013;88(6):321-7. doi: 10.1266/ggs.88.321.
Chromosome mutations occur in common wheat carrying a monosome of gametocidal (Gc) chromosomes 2C and 3C(SAT). These Gc chromosomes have been known to induce chromosomal breakage in a rye chromosome 1R added to common wheat. We attempted to introduce the two Gc chromosomes into the other six rye chromosome (2R to 7R) addition or substitution lines of common wheat to establish a set of chromosomal rearrangement-inducing lines for rye chromosomes. We obtained critical plants that had a pair of rye chromosomes and one Gc chromosome for 2R, 3R, 4R and 6R, and semi-critical plants that were monotelodisomic and monosomic for 5R. Chromosomal aberrations are expected to occur in the progeny of these plants. Besides we established self-fertile disomic 2C addition lines of common wheat that were disomic substitution for 3R, disomic addition for 6R, monotelodisomic for 5R, and monosomic for 7R. We can produce the critical plants of the respective rye chromosomes by crossing above lines to the respective wheat-rye disomic addition or substitution lines. During the cytological screening in this study, we found Gc-induced chromosomal aberrations for every rye chromosome. The stocks reported here can be used to produce dissection lines for each of the rye chromosomes in common wheat by the Gc system.
携带杀配子（Gc）染色体2C和3C（随体）单体的普通小麦中发生了染色体突变。已知这些Gc染色体可诱导添加到普通小麦中的黑麦染色体1R发生染色体断裂。我们试图将这两条Gc染色体导入普通小麦的其他六条黑麦染色体（2R至7R）附加系或代换系中，以建立一套用于黑麦染色体的染色体重排诱导系。我们获得了关键植株，其具有一对黑麦染色体和一条针对2R、3R、4R和6R的Gc染色体，以及半关键植株，其对于5R是单端二体和单体的。预计这些植株的后代会发生染色体畸变。此外，我们建立了普通小麦的可育二体2C附加系，它们对于3R是二体代换，对于6R是二体附加，对于5R是单端二体，对于7R是单体。通过将上述品系与各自的小麦-黑麦二体附加或代换系杂交，我们可以产生各个黑麦染色体的关键植株。在本研究的细胞学筛选过程中，我们发现每条黑麦染色体都有Gc诱导的染色体畸变。本文报道的材料可用于通过Gc系统产生普通小麦中每条黑麦染色体的剖析系。
