Gänshirt-Ahlert D, Burschyk M, Garritsen H S, Helmer L, Miny P, Horst J, Schneider H P, Holzgreve W
Department of Obstetrics and Gynecology, University of Münster, Germany.
Am J Obstet Gynecol. 1992 May;166(5):1350-5. doi: 10.1016/0002-9378(92)91603-8.
We wanted to test whether the recently described method of using the transferrin receptor system for fluorescence-activated cell-sorter enrichment of nucleated red blood cells can be used for prenatal diagnosis from maternal blood.
Instead of the laborious, expensive fluorescence-activated cell-sorter system, we used the newly described magnetic-activated cell sorter.
An effective enrichment could be achieved with separation of lymphocyte subsets. With the transferrin receptor, however, the enrichment was very inefficient because of the poor specificity of the antibody itself. Even in umbilical cord blood only 25% of nucleated red blood cells were labeled as demonstrated by immunogold silver enhancement of transferrin receptor-labeled cells.
In spite of the availability of a fast and effective separation method (magnetic-activated cell sorter) the use of the transferrin receptor antigen alone is not likely to enable a reliable identification of fetal cells in maternal circulation.
我们想要测试最近描述的利用转铁蛋白受体系统通过荧光激活细胞分选仪富集有核红细胞的方法是否可用于从母血中进行产前诊断。
我们没有使用费力且昂贵的荧光激活细胞分选仪系统，而是采用了新描述的磁激活细胞分选仪。
通过分离淋巴细胞亚群可实现有效的富集。然而，对于转铁蛋白受体，由于抗体本身特异性较差，富集效率非常低。即使在脐带血中，通过转铁蛋白受体标记细胞的免疫金银增强法显示，只有25%的有核红细胞被标记。
尽管有快速有效的分离方法（磁激活细胞分选仪），但仅使用转铁蛋白受体抗原不太可能可靠地识别母血循环中的胎儿细胞。
