单细胞分析揭示微嵌合体的生物学秘密。
Unravelling the biological secrets of microchimerism by single-cell analysis.
机构信息
Sahlgrenska Cancer Center, Department of Pathology and Genetics, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Medicinaregatan 1F, Gothenburg, Sweden.
Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Harrachgasse 21, Graz, Austria.
出版信息
Brief Funct Genomics. 2018 Jul 1;17(4):255-264. doi: 10.1093/bfgp/elx027.
The presence of microchimeric cells is known for >100 years and well documented since decades. Earlier, microchimeric cells were mainly used for cell-based non-invasive prenatal diagnostics during early pregnancy. Microchimeric cells are also present beyond delivery and are associated to various autoimmune diseases, tissue repair, cancer and immune tolerance. All these findings were based on low complexity studies and occasionally accompanied by artefacts not allowing the biological functions of microchimerism to be determined. However, with the recent developments in single-cell analysis, new means to identify and characterize microchimeric cells are available. Cell labelling techniques in combination with single-cell analysis provide a new toolbox to decipher the biology of microchimeric cells at molecular and cellular level. In this review, we discuss how recent developments in single-cell analysis can be applied to determine the role and function of microchimeric cells.
微嵌合细胞的存在已被人们认识>100 年，并在过去几十年中有了充分的记录。早期，微嵌合细胞主要用于妊娠早期的基于细胞的非侵入性产前诊断。微嵌合细胞在分娩后也存在，并与各种自身免疫性疾病、组织修复、癌症和免疫耐受有关。所有这些发现都是基于低复杂度的研究，偶尔会伴有不允许确定微嵌合作用的生物学功能的假象。然而，随着单细胞分析的最新发展，现在有了新的方法来识别和描述微嵌合细胞。细胞标记技术与单细胞分析相结合，为在分子和细胞水平上破译微嵌合细胞的生物学特性提供了一个新的工具箱。在这篇综述中，我们讨论了单细胞分析的最新发展如何应用于确定微嵌合细胞的作用和功能。
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