El Kirat K, Burton I, Dupres V, Dufrene Y F
Unité de chimie des interfaces, Université catholique de Louvain, Belgium.
J Microsc. 2005 Jun;218(Pt 3):199-207. doi: 10.1111/j.1365-2818.2005.01480.x.
Since the late 1980s, atomic force microscopy (AFM) has been increasingly used in biological sciences and it is now established as a versatile tool to address the structure, properties and functions of biological specimens. AFM is unique in that it provides three-dimensional images of biological structures, including biomolecules, lipid films, 2D protein crystals and cells, under physiological conditions and with unprecedented resolution. A crucial prerequisite for successful, reliable biological AFM is that the samples need to be well attached to a solid substrate using appropriate, nondestructive methods. In this review, we discuss common techniques for immobilizing biological specimens for AFM studies.
自20世纪80年代末以来，原子力显微镜（AFM）在生物科学中的应用越来越广泛，如今已成为研究生物样本结构、性质和功能的多功能工具。AFM的独特之处在于，它能够在生理条件下以前所未有的分辨率提供生物结构的三维图像，包括生物分子、脂质膜、二维蛋白质晶体和细胞。成功、可靠地进行生物AFM的一个关键前提是，需要使用适当的、无损的方法将样品良好地附着在固体基质上。在这篇综述中，我们讨论了用于固定生物样本以进行AFM研究的常用技术。
