Vesenka J, Mosher C, Schaus S, Ambrosio L, Henderson E
Iowa State University, Ames, USA.
Biotechniques. 1995 Aug;19(2):240-8, 849, 852-3.
The atomic force microscope (AFM), a three-dimensional imaging tool that can measure structures from the atomic level to micron scale, has been combined with an inverted optical microscope capable of confocal imaging. The robust design of this microscope, termed the BioScope, enables the operator to use fluorescent markers on a wide variety of biological specimens to determine internal structure to 200 nm resolution and determine surface morphology of the same sample to 20 nm resolution while imaging under physiological conditions. In this report we demonstrate the capabilities of the BioScope by examining living Xenopus retinal glial (XR1) cells, Drosophila polytene chromosomes and colloidal gold-labeled plasmid DNA.
原子力显微镜（AFM）是一种三维成像工具，能够测量从原子水平到微米尺度的结构，它已与一台具备共聚焦成像能力的倒置光学显微镜相结合。这种被称为BioScope的显微镜设计坚固，使操作人员能够在多种生物样本上使用荧光标记，在生理条件下成像时，以200纳米的分辨率确定内部结构，并以20纳米的分辨率确定同一样本的表面形态。在本报告中，我们通过检查非洲爪蟾视网膜神经胶质（XR1）细胞、果蝇多线染色体和胶体金标记的质粒DNA来展示BioScope的功能。
