Oh Jeonghun, Ryu Jea Sung, Lee Moosung, Jung Jaehwang, Han SeungYun, Chung Hyun Jung, Park Yongkeun
Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, South Korea.
KAIST Institute for Health Science and Technology, KAIST, Daejeon 34141, South Korea.
Biomed Opt Express. 2020 Feb 3;11(3):1257-1267. doi: 10.1364/BOE.377740. eCollection 2020 Mar 1.
Measuring alterations in bacteria upon antibiotic application is important for basic studies in microbiology, drug discovery, clinical diagnosis, and disease treatment. However, imaging and 3D time-lapse response analysis of individual bacteria upon antibiotic application remain largely unexplored mainly due to limitations in imaging techniques. Here, we present a method to systematically investigate the alterations in individual bacteria in 3D and quantitatively analyze the effects of antibiotics. Using optical diffraction tomography, responses of and to various concentrations of ampicillin were investigated in a label-free and quantitative manner. The presented method reconstructs the dynamic changes in the 3D refractive-index distributions of living bacteria in response to antibiotics at sub-micrometer spatial resolution.
测量抗生素作用下细菌的变化对于微生物学基础研究、药物发现、临床诊断和疾病治疗都很重要。然而，由于成像技术的限制，抗生素作用下单个细菌的成像及三维延时响应分析在很大程度上仍未得到充分探索。在此，我们提出一种方法，用于系统地研究单个细菌的三维变化并定量分析抗生素的作用效果。利用光学衍射层析成像技术，以无标记和定量的方式研究了大肠杆菌和金黄色葡萄球菌对不同浓度氨苄青霉素的响应。所提出的方法以亚微米空间分辨率重建了活细菌三维折射率分布响应抗生素的动态变化。
