Shain D H, Yoo J, Slaughter R G, Hayes S E, Ji T H
Department of Molecular Biology, University of Wyoming, Laramie 82071-3944.
Anal Biochem. 1992 Jan;200(1):47-51. doi: 10.1016/0003-2697(92)90275-c.
Electrofractionation (EF) is a technique that allows electrophoretic materials to be detected and recovered following electrophoresis. The EF apparatus utilizes the resolving power of electrophoresis and the mobile phase of liquid chromatography to create a continuous elution system. Our data indicate that EF is able to detect and recover oligonucleotides, as DNA fragments, proteins, and presumably other material that can be analyzed by electrophoresis. EF shares functional similarities with high-performance electrophoresis chromatography (HPEC) but operates by a different strategy and at a fraction of the cost. Moreover, EF can be constructed largely from standard laboratory equipment. The simplicity, rapid analysis times, low cost, and high recovery yields of EF make this system a practical alternative to the conventional detection and purification methods used for biomolecules.
电分馏（EF）是一种能在电泳后对电泳材料进行检测和回收的技术。EF仪器利用电泳的分辨能力和液相色谱的流动相来创建一个连续洗脱系统。我们的数据表明，EF能够检测和回收作为DNA片段的寡核苷酸、蛋白质以及大概其他可通过电泳分析的物质。EF与高效电泳色谱法（HPEC）有功能上的相似之处，但采用不同的策略运行，且成本仅为其一小部分。此外，EF在很大程度上可以由标准实验室设备构建而成。EF的简单性、快速分析时间、低成本和高回收率使得该系统成为用于生物分子的传统检测和纯化方法的一种实用替代方案。
