Gumieniczek Anna
Department of Medicinal Chemistry, Medical University of Lublin, Chodźki Str. 6, 20-093, Lublin, Poland.
J Biomed Sci. 2005;12(3):531-7. doi: 10.1007/s11373-005-6733-2.
The study was undertaken to analyze the effect of pioglitazone on superoxide dismutase (Cu, Zn-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R), glutathione (GSH), ascorbic acid (AA), lipid peroxidation products (LPO) and protein carbonyl groups (PCG) in the heart of alloxan-induced diabetic rabbits after 4 and 8 weeks of pioglitazone treatment. In diabetic animals, Cu, Zn-SOD and CAT were elevated by 60 and 55%, and 90 and 77% as compared to controls at 4 and 8 weeks, respectively. GSH-Px, GSSG-R and GSH were diminished by 11, 14 and 33% as compared to controls at 4 or 8 weeks. AA was diminished by 52 and 41%. At P <0.05, pioglitazone normalized the activities of Cu, Zn-SOD, GSH-Px and GSSG-R. The activity of CAT was modified as compared to diabetic non-treated rabbits. After pioglitazone treatment, GSH and AA were increased as compared to diabetic non-treated animals. In diabetic rabbits, LPO was elevated by 52 and 111% and normalized by pioglitazone treatment. PCG was elevated by 72 and 133% and diminished as compared to diabetic non-treated animals at 8 weeks. The study shows that pioglitazone reduces oxidative stress in the heart of diabetic rabbits. In therapy, similar action can improve the cardiovascular system of diabetic patients.
本研究旨在分析吡格列酮治疗4周和8周后，对四氧嘧啶诱导的糖尿病兔心脏中超氧化物歧化酶（铜锌超氧化物歧化酶）、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽、抗坏血酸、脂质过氧化产物和蛋白质羰基基团的影响。在糖尿病动物中，与对照组相比，4周和8周时铜锌超氧化物歧化酶和过氧化氢酶分别升高了60%和55%，以及90%和77%。4周或8周时，谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽与对照组相比分别降低了11%、14%和33%。抗坏血酸降低了52%和41%。在P<0.05时，吡格列酮使铜锌超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性恢复正常。与未治疗的糖尿病兔相比，过氧化氢酶的活性有所改变。吡格列酮治疗后，与未治疗的糖尿病动物相比，谷胱甘肽和抗坏血酸增加。在糖尿病兔中，脂质过氧化产物升高了52%和111%，经吡格列酮治疗后恢复正常。蛋白质羰基基团升高了72%和133%，在8周时与未治疗的糖尿病动物相比有所降低。该研究表明，吡格列酮可减轻糖尿病兔心脏中的氧化应激。在治疗中，类似的作用可改善糖尿病患者的心血管系统。
