Prabhakar Pankaj, Reeta K H, Maulik S K, Dinda A K, Gupta Y K
Department of Pharmacology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Eur J Nutr. 2015 Oct;54(7):1117-27. doi: 10.1007/s00394-014-0788-7. Epub 2014 Oct 28.
Thymoquinone (TQ), a bioactive constituent of Nigella sativa (Linn.) seed, which is commonly used as a spice in Asian food, has been reported to possess a wide range of biological effects. The present study evaluated the effect of TQ on high-fructose diet (HFD)-induced metabolic syndrome (MetS) in male Wistar rats.
MetS was induced by 60% HFD over 42 days. TQ (25, 50 and 100 mg/kg, p.o. once daily) was administered along with HFD for 42 days. Pioglitazone (10 mg/kg, p.o. once daily) was used as a standard drug. Plasma glucose, triglycerides, total cholesterol and HDL-cholesterol were estimated on days 0 and 42. Change in blood pressure, oral glucose tolerance and insulin resistance were measured. Hepatic thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase levels were estimated as measures of hepatic oxidative stress. Hepatic mRNA of PPAR-α and PPAR-γ was also studied.
TQ prevented the characteristic features of HFD-induced MetS, such as hyperglycaemia, hypertriglyceridemia, hypercholesterolaemia and elevated systolic blood pressure. TQ also prevented impaired glucose tolerance and insulin resistance. It also ameliorated HFD-induced increase in hepatic TBARS and depletion of SOD, catalase and GSH. TQ prevented reduction in hepatic mRNA of PPAR-α and PPAR-γ in HFD rats, and the effects were comparable to those of pioglitazone.
This study demonstrates protective effect of TQ against HFD-induced MetS on rats which might have been mediated via PPAR mechanism.
百里醌（TQ）是黑种草籽中的一种生物活性成分，在亚洲食品中常用作香料，据报道具有广泛的生物学效应。本研究评估了TQ对雄性Wistar大鼠高糖饮食（HFD）诱导的代谢综合征（MetS）的影响。
通过60%的HFD喂养42天诱导MetS。TQ（25、50和100mg/kg，口服，每日一次）与HFD一起给药42天。吡格列酮（10mg/kg，口服，每日一次）用作标准药物。在第0天和第42天测定血浆葡萄糖、甘油三酯、总胆固醇和高密度脂蛋白胆固醇。测量血压变化、口服葡萄糖耐量和胰岛素抵抗。估计肝组织中硫代巴比妥酸反应性物质（TBARS）、还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶水平，作为肝氧化应激的指标。还研究了肝组织中PPAR-α和PPAR-γ的mRNA。
TQ可预防HFD诱导的MetS的特征，如高血糖、高甘油三酯血症、高胆固醇血症和收缩压升高。TQ还可预防葡萄糖耐量受损和胰岛素抵抗。它还改善了HFD诱导的肝组织TBARS增加以及SOD、过氧化氢酶和GSH的消耗。TQ可预防HFD大鼠肝组织中PPAR-α和PPAR-γ mRNA的减少，其效果与吡格列酮相当。
本研究表明TQ对大鼠HFD诱导的MetS具有保护作用，这可能是通过PPAR机制介导的。
