Aziz Sina, Anjum Shazia, Rehman Atta Ur, Akram Durre Samin, Naqvi Syed Ali Anwar, Rizvi Syed Adibul Hasan
Department of Pediatrics, Sindh Institute of Urology and Transplantation, Karachi.
J Pak Med Assoc. 2005 May;55(5):188-92.
To determine the pattern of excretion of total bilirubin IXa and IXb in the first meconium of newborn infants.
First two newborns of varying gestational age were selected every week through random sampling from the neonatal unit. Of the 41 newborn infants selected 8 expired before meconium passage, hence the results are from 33 newborns. Meconium was collected and stored at -20 degrees C, protected by aluminium foil. Samples were defrosted, vortex mixed with equal amount of dimethyl-sulfoxide, centrifuged, and analyzed by HPLC.
Unconjugated Bilirubin-IXalpha and -IXbeta were identified and quantitative estimation of Bilirubin-IXa done. Bilirubin-IXb was greater than 50% of the total, in the first meconium of the newborn. Amount of bilirubin excreted in meconium was 29.2 - 90.8 mg [0.051 - 0.155 mmol] per sample of meconium passed. Amount was 9.7 mg/ Kg of body weight in term newborn and 12 mg/kg in preterm.
The amount of bilirubin -IXb decreases and bilirubin-IXa increases with increasing gestational age. Newborns with birth asphyxia (BA) had significantly greater quantity of bilirubin in meconium, compared to infants without BA.
确定新生儿首次胎粪中总胆红素IXa和IXb的排泄模式。
每周从新生儿病房随机抽取两名不同胎龄的新生儿。在选取的41名新生儿中，8名在胎粪排出前死亡，因此结果来自33名新生儿。收集胎粪并储存在-20℃，用铝箔保护。将样本解冻，与等量二甲基亚砜涡旋混合，离心，并用高效液相色谱法分析。
鉴定出未结合胆红素-IXα和-IXβ，并对胆红素-IXa进行了定量测定。在新生儿的首次胎粪中，胆红素-IXb占总量的50%以上。每次排出的胎粪样本中胆红素排泄量为29.2 - 90.8毫克[0.051 - 0.155毫摩尔]。足月儿胎粪中胆红素排泄量为9.7毫克/千克体重，早产儿为12毫克/千克体重。
随着胎龄增加，胆红素-IXb含量降低，胆红素-IXa含量增加。与无出生窒息的婴儿相比，出生窒息的新生儿胎粪中的胆红素含量显著更高。
