Timm A E, Pringle K L, Warnich L
Department of Entomology and Centre for Agricultural Biodiversity, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa.
Bull Entomol Res. 2005 Jun;95(3):187-91. doi: 10.1079/ber2004348.
The woolly apple aphid Eriosoma lanigerum (Hausmann) is one of the most damaging apple pests in South Africa. Information on its genetic diversity is lacking and this study, in which the genetic structure of parthenogenetic E. lanigerum populations was characterized in the Western Cape Province of South Africa, represents the first local study of its kind. A total of 192 individuals from four different regions were collected and analysed using amplified fragment length polymorphism (AFLP). Using five selective AFLP primer pairs, 250 fragments were scored for analysis. Results indicated that a low level of genetic variation was apparent in E. lanigerum populations in the Western Cape (H = 0.0192). Furthermore, populations collected from geographically distant regions were very closely related, which can partly be explained by the fact that agricultural practices were responsible for dissemination of populations from a common ancestor to geographically distant areas. The low level of variation found indicated that the possibility of controlling E. lanigerum in the Western Cape using host plant resistance is favourable. This is the first report of AFLP being used to characterize the genetic structure of an aphid species. Results indicate that this marker may be useful for analysis of other aphid species.
苹果绵蚜Eriosoma lanigerum（豪斯曼）是南非最具危害性的苹果害虫之一。目前缺乏关于其遗传多样性的信息，而本研究对南非西开普省孤雌生殖的苹果绵蚜种群的遗传结构进行了表征，是此类研究的首次本地研究。从四个不同地区共收集了192个个体，并使用扩增片段长度多态性（AFLP）进行分析。使用五对选择性AFLP引物对，共分析了250个片段。结果表明，西开普省的苹果绵蚜种群中明显存在低水平的遗传变异（H = 0.0192）。此外，从地理上相距遥远的地区收集的种群关系非常密切，这部分可以解释为农业实践导致了种群从共同祖先传播到地理上遥远的地区。所发现的低水平变异表明，利用寄主植物抗性控制西开普省苹果绵蚜的可能性很大。这是首次报道使用AFLP来表征蚜虫物种的遗传结构。结果表明，该标记可能对分析其他蚜虫物种有用。
