López-Molina Dorotea, Navarro-Martínez María Dolores, Rojas Melgarejo Francisco, Hiner Alexander N P, Chazarra Soledad, Rodríguez-López José Neptuno
Grupo de Investigación de Enzimología, Departamento de Bioquímica y Biología Molecular A, Facultad de Biología, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain.
Phytochemistry. 2005 Jun;66(12):1476-84. doi: 10.1016/j.phytochem.2005.04.003.
A high molecular weight inulin has been prepared from artichoke (Cynara scolymus L.) agroindustrial wastes using environmentally benign aqueous extraction procedures. Physico-chemical analysis of the properties of artichoke inulin was carried out. Its average degree of polymerization was 46, which is higher than for Jerusalem artichoke, chicory, and dahlia inulins. GC-MS confirmed that the main constituent monosaccharide in artichoke inulin was fructose and its degradation by inulinase indicated that it contained the expected beta-2,1-fructan bonds. The FT-IR spectrum was identical to that of chicory inulin. These data indicate that artichoke inulin will be suitable for use in a wide range of food applications. The health-promoting prebiotic effects of artichoke inulin were demonstrated in an extensive microbiological study showing a long lasting bifidogenic effect on Bifidobacterium bifidum ATCC 29521 cultures and also in mixed cultures of colonic bacteria.
已采用环境友好型水相提取工艺，从洋蓟（Cynara scolymus L.）农业工业废弃物中制备出一种高分子量菊粉。对洋蓟菊粉的性质进行了物理化学分析。其平均聚合度为46，高于菊芋、菊苣和大丽花菊粉。气相色谱-质谱联用（GC-MS）证实，洋蓟菊粉中的主要单糖成分是果糖，其被菊粉酶降解表明它含有预期的β-2,1-果聚糖键。傅里叶变换红外光谱（FT-IR）与菊苣菊粉的光谱相同。这些数据表明，洋蓟菊粉适用于广泛的食品应用。在一项广泛的微生物学研究中，证实了洋蓟菊粉具有促进健康的益生元作用，该研究表明其对双歧双歧杆菌ATCC 29521培养物具有持久的双歧增殖作用，对结肠细菌混合培养物也有此作用。
