Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
CRC Materiali Polimerici (LaMPo), Department of Chemistry, University of Milan, via Golgi 19, 20133 Milan, Italy.
Biomed Res Int. 2019 Sep 25;2019:1083952. doi: 10.1155/2019/1083952. eCollection 2019.
Globe artichoke is an intriguing source of indigestible sugar polymers such as inulin-type fructans. In this study, the effect of ultrasound in combination with ethanol precipitation to enhance the extraction of long chain fructans from artichoke wastes has been evaluated. The inulin-type fructans content both from bracts and stems was measured using an enzymatic fructanase-based assay, while its average degree of polymerization (DP) was determined by HPLC-RID analysis. Results show that this method provides artichoke extracts with an inulin-type fructans content of 70% with an average DP between 32 and 42 both in bracts and in stems. The prebiotic effect of long chain inulins from artichoke extract wastes was demonstrated by its ability to support the growth of five and four species, previously characterized as probiotics. Besides, we considered the possibility to industrialize the process developing a simpler method for the production of inulin-type fructans from the artichoke wastes so that the artichoke inulin preparation could be suitable for its use in synbiotic formulations in combination with different probiotics for further studies including trials.
朝鲜蓟是一种有趣的不可消化糖聚合物（如菊粉型果聚糖）来源。在这项研究中，评估了超声结合乙醇沉淀来增强从朝鲜蓟废物中提取长链果聚糖的效果。使用基于酶菊粉酶的测定法测量了苞片和茎中的菊粉型果聚糖含量，而其平均聚合度（DP）则通过 HPLC-RID 分析确定。结果表明，该方法为朝鲜蓟提取物提供了菊粉型果聚糖含量为 70%的提取物，其在苞片和茎中的平均 DP 在 32 到 42 之间。从朝鲜蓟提取物废物中提取的长链菊粉的益生元作用通过其支持五种 和四种 先前被表征为益生菌的生长的能力来证明。此外，我们考虑了通过开发一种从朝鲜蓟废物中生产菊粉型果聚糖的更简单方法来实现工业化的可能性，以便朝鲜蓟菊粉制剂可以与不同的益生菌结合用于进一步研究，包括临床试验。
