Nielsen Peter R, Nietlispach Daniel, Buscaino Alessia, Warner Rosemary J, Akhtar Asifa, Murzin Alexey G, Murzina Natalia V, Laue Ernest D
Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK.
J Biol Chem. 2005 Sep 16;280(37):32326-31. doi: 10.1074/jbc.M501347200. Epub 2005 Jun 17.
We report here the structure of the putative chromo domain from MOF, a member of the MYST family of histone acetyltransferases that acetylates histone H4 at Lys-16 and is part of the dosage compensation complex in Drosophila. We found that the structure of this domain is a beta-barrel that is distinct from the alpha + beta fold of the canonical chromo domain. Despite the differences, there are similarities that support an evolutionary relationship between the two domains, and we propose the name "chromo barrel." The chromo barrel domains may be divided into two groups, MSL3-like and MOF-like, on the basis of whether a group of conserved aromatic residues is present or not. The structure suggests that, although the MOF-like domains may have a role in RNA binding, the MSL3-like domains could instead bind methylated residues. The MOF chromo barrel shares a common fold with other chromatin-associated modules, including the MBT-like repeat, Tudor, and PWWP domains. This structural similarity suggests a probable evolutionary pathway from these other modules to the canonical chromo domains (or vice versa) with the chromo barrel domain representing an intermediate structure.
我们在此报告MOF假定的染色体结构域，MOF是组蛋白乙酰转移酶MYST家族的成员，可在赖氨酸16位点乙酰化组蛋白H4，并且是果蝇剂量补偿复合物的一部分。我们发现该结构域的结构是一个β桶，与典型染色体结构域的α + β折叠不同。尽管存在差异，但仍有相似之处支持这两个结构域之间的进化关系，我们提出了“染色体桶”这一名称。根据一组保守芳香族残基是否存在，染色体桶结构域可分为两组，即MSL3样和MOF样。该结构表明，虽然MOF样结构域可能在RNA结合中起作用，但MSL3样结构域可能反而结合甲基化残基。MOF染色体桶与其他染色质相关模块具有共同的折叠结构，包括MBT样重复序列、Tudor结构域和PWWP结构域。这种结构相似性表明，从这些其他模块到典型染色体结构域（反之亦然）可能存在一条进化途径，其中染色体桶结构域代表一种中间结构。
