Yamada M, Mochizuki H, Kawai M, Tsubota K, Bryce T J
Department of Ophthalmology, Keigo University School of Medicine, Tokyo, Japan.
Br J Ophthalmol. 2005 Jul;89(7):803-5. doi: 10.1136/bjo.2004.055822.
BACKGROUND/AIM: Recent studies have demonstrated that tear lipocalin (TL) and phospholipids have a crucial role in maintaining tear film stability. The level of TL in patients with meibomian gland dysfunction (MGD) was examined and these data were correlated with the severity of their clinical disorder.
12 patients with obstructive MGD, 12 patients with seborrhoeic MGD, and 12 age matched normal control subjects participated in this study. 3 mul of unstimulated tears were collected with a micropipette from the inferior tear meniscus in the right eye of all subjects. Tear samples were fractionated by high performance liquid chromatography, and TL concentrations were assayed with a bicinchoninic acid technique.
The mean concentration of TL in patients with obstructive and seborrhoeic MGD was significantly lower than in normal controls. TL concentration correlated positively with tear film break up time and negatively with fluorescein staining scores.
These results suggest that TL deficiency may be a predisposing factor for the manifestation of symptoms in MGD.
背景/目的：近期研究表明，泪液脂钙蛋白（TL）和磷脂在维持泪膜稳定性方面起着关键作用。本研究检测了睑板腺功能障碍（MGD）患者的TL水平，并将这些数据与他们临床病症的严重程度相关联。
12例阻塞性MGD患者、12例脂溢性MGD患者以及12例年龄匹配的正常对照者参与了本研究。用微量移液器从所有受试者右眼下方泪液弯月面收集3微升未刺激的泪液。泪液样本通过高效液相色谱法进行分离，并用二喹啉甲酸技术测定TL浓度。
阻塞性和脂溢性MGD患者的TL平均浓度显著低于正常对照组。TL浓度与泪膜破裂时间呈正相关，与荧光素染色评分呈负相关。
这些结果表明，TL缺乏可能是MGD症状表现的一个易感因素。
