Jiang Tong, Zhou Xueping
Institute of Biotechnology, Zhejiang University, 310029, Hangzhou, People's Republic of China.
Virus Genes. 2005 Aug;31(1):43-8. doi: 10.1007/s11262-004-2198-z.
A virus isolate Y160 was obtained from Malvastrum coromandelianum showing yellow vein symptoms in Baoshan, Yunnan province of China. The complete nucleotide sequence of DNA-A was determined, it contains 2747 nucleotides and has typical genomic organization of a begomovirus. Comparisons show that the total DNA-A of Y160 has the highest sequence identity (82.2%) with that of Malvastrum yellow vein virus-[Y47] (AJ457824), while less than 77.6% identities are found when compared with the other begomoviruses. The molecular data show that Y160 is a distinct begomovirus species, for which the name Malvastrum yellow vein Yunnan virus (MYVYNV) is proposed. Satellite DNA molecule (Y160beta) was found to be associated with Y160 using the abutting primers beta 0l and beta 02 specific for DNA beta. Y160beta consists of 1350 nucleotides, with a functional ORF (beta C1) in complementary-sense DNA. Y160beta has the highest sequence identity (73.5%) with DNA beta molecule associated with Malvastrum yellow vein virus-[Y47] (AJ421482).
从中国云南省保山市表现出黄脉症状的赛葵上获得了病毒分离物Y160。测定了DNA-A的完整核苷酸序列，其包含2747个核苷酸，具有双生病毒典型的基因组结构。比较表明，Y160的DNA-A全长与赛葵黄脉病毒-[Y47]（AJ457824）的序列同一性最高（82.2%），而与其他双生病毒相比，同一性低于77.6%。分子数据表明，Y160是一个独特的双生病毒种，为此提出了赛葵黄脉云南病毒（MYVYNV）这一名称。使用对DNAβ特异的邻接引物β01和β02发现卫星DNA分子（Y160β）与Y160相关联。Y160β由1350个核苷酸组成，在互补链DNA中有一个功能性开放阅读框（βC1）。Y160β与和赛葵黄脉病毒-[Y47]相关联的DNAβ分子（AJ421482）的序列同一性最高（73.5%）。
