Guo Wei, Jiang Tong, Zhang Xian, Li Guixin, Zhou Xueping
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China.
Appl Environ Microbiol. 2008 Mar;74(6):1909-13. doi: 10.1128/AEM.02461-07. Epub 2008 Feb 1.
Previous studies have found that the diversity of begomovirus-associated DNA beta satellites is related to host and geographical origin. In this study, we have cloned and sequenced 20 different isolates of DNA beta molecules associated with Malvastrum yellow vein virus (MYVV) isolated from Malvastrum coromandelianum plants in different geographical locations of Yunnan Province, China. Analyses of their molecular variation indicate that the satellites are clustered together according to their geographical location but that they have only limited sequence diversity. Infectivity tests using infectious clones of MYVV and its associated DNA beta molecule indicate that MYVV DNA beta is indispensable for symptom induction in Nicotiana benthamiana, N. glutinosa, Petunia hybrida, and M. coromandelianum plants. Furthermore, we showed that MYVV interacts functionally with heterologous DNA beta molecules in N. benthamiana plants.
先前的研究发现，双生病毒相关的DNAβ卫星的多样性与宿主和地理起源有关。在本研究中，我们克隆并测序了20种不同的与云南不同地理位置的赛葵黄脉病毒（MYVV）相关的DNAβ分子分离株，这些分离株来自中国云南省不同地理位置的黄葵植株。对它们的分子变异分析表明，卫星根据其地理位置聚集在一起，但它们的序列多样性有限。使用MYVV及其相关DNAβ分子的感染性克隆进行的感染性测试表明，MYVV DNAβ对于本氏烟草、黏毛烟草、矮牵牛和黄葵植株中的症状诱导是必不可少的。此外，我们表明MYVV在本氏烟草植株中与异源DNAβ分子发生功能相互作用。
