Aimbire F, Albertine R, de Magalhães R G, Lopes-Martins R A B, Castro-Faria-Neto H C, Zângaro R A, Chavantes M C, Pacheco M T T
Laboratório de Farmacologia e LASER, Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba Av: Shishima Hifumi 2911, São José dos Campos, S.P., 12240-000, Brazil.
Lasers Med Sci. 2005;20(1):11-20. doi: 10.1007/s10103-005-0339-9. Epub 2005 Jun 18.
The purpose of this study was to investigate the effect of low level laser therapy (LLLT) on male Wistar rat trachea hyperreactivity (RTHR), bronchoalveolar lavage (BAL) and lung neutrophils influx after Gram-negative bacterial lipopolyssacharide (LPS) intravenous injection. The RTHR, BAL and lung neutrophils influx were measured over different intervals of time (90 min, 6 h, 24 h and 48 h). The energy density (ED) that produced an anti-inflammatory effect was 2.5 J/cm(2), reducing the maximal contractile response and the sensibility of trachea rings to methacholine after LPS. The same ED produced an anti-inflammatory effect on BAL and lung neutrophils influx. The Celecoxib COX-2 inhibitor reduced RTHR and the number of cells in BAL and lung neutrophils influx of rats treated with LPS. Celecoxib and LLLT reduced the PGE(2) and TXA(2) levels in the BAL of LPS-treated rats. Our results demonstrate that LLLT produced anti-inflammatory effects on RTHR, BAL and lung neutrophils influx in association with inhibition of COX-2-derived metabolites.
本研究的目的是探讨低强度激光疗法（LLLT）对革兰氏阴性菌脂多糖（LPS）静脉注射后雄性Wistar大鼠气管高反应性（RTHR）、支气管肺泡灌洗（BAL）和肺中性粒细胞浸润的影响。在不同时间间隔（90分钟、6小时、24小时和48小时）测量RTHR、BAL和肺中性粒细胞浸润情况。产生抗炎作用的能量密度（ED）为2.5 J/cm(2)，可降低LPS后气管环对乙酰甲胆碱的最大收缩反应和敏感性。相同的ED对BAL和肺中性粒细胞浸润产生抗炎作用。塞来昔布COX-2抑制剂降低了LPS处理大鼠的RTHR以及BAL中的细胞数量和肺中性粒细胞浸润。塞来昔布和LLLT降低了LPS处理大鼠BAL中的PGE(2)和TXA(2)水平。我们的结果表明，LLLT对RTHR、BAL和肺中性粒细胞浸润产生抗炎作用，同时抑制COX-2衍生代谢产物。
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