Wingren Christer, Borrebaeck Carl A K
Department of Immunotechnology, Lund University, PO Box 7031, Lund, Sweden.
Expert Rev Proteomics. 2004 Oct;1(3):355-64. doi: 10.1586/14789450.1.3.355.
Antibody-based microarrays are a novel technology that hold great promise in proteomics. Microarrays can be printed with thousands of recombinant antibodies carrying the desired specificities, the biologic sample (e.g., an entire proteome) and any specifically bound analytes detected. The microarray patterns that are generated can then be converted into proteomic maps, or molecular fingerprints, revealing the composition of the proteome. Using this tool, global proteome analysis and protein expression profiling will thus provide new opportunities for biomarker discovery, drug target identification and disease diagnostics, as well as providing insights into disease biology. Intense work is currently underway to develop this novel technology platform into the high-throughput proteomic tool required by the research community.
基于抗体的微阵列是一种在蛋白质组学领域极具前景的新技术。微阵列可以打印上数千种具有所需特异性的重组抗体，用于检测生物样品（例如，整个蛋白质组）和任何特异性结合的分析物。然后，所生成的微阵列模式可以转换为蛋白质组图谱或分子指纹，揭示蛋白质组的组成。利用这一工具，全局蛋白质组分析和蛋白质表达谱分析将为生物标志物发现、药物靶点识别和疾病诊断提供新的机会，同时也有助于深入了解疾病生物学。目前，人们正在进行大量工作，将这一新技术平台发展成为研究界所需的高通量蛋白质组学工具。
