Lv Lin-Li, Liu Bi-Cheng
Institute of Nephrology, Zhong Da Hospital, Southeast University, Nanjing, China.
Expert Rev Proteomics. 2007 Aug;4(4):505-13. doi: 10.1586/14789450.4.4.505.
The antibody microarray is an intrinsically robust and quantitative system that delivers high-throughput and parallel measurements on particular sets of known proteins. It has become an important proteomics research tool, complementary to the conventional unbiased separation-based and mass spectrometry-based approaches. This review summarizes the technical aspects of production and the application for quantitative proteomic analysis with an emphasis on disease proteomics, especially the identification of biomarkers. Quality control, data analysis methods and the challenges for quantitative assays are also discussed.
抗体微阵列是一种本质上强大且定量的系统，可对特定已知蛋白质组进行高通量并行测量。它已成为一种重要的蛋白质组学研究工具，是对传统的基于无偏分离和基于质谱的方法的补充。本综述总结了其生产的技术方面以及在定量蛋白质组分析中的应用，重点是疾病蛋白质组学，特别是生物标志物的鉴定。还讨论了质量控制、数据分析方法以及定量分析面临的挑战。
