Ware Joy L
Department of Pathology, Virginia Commonwealth University School of Medicine, Box 980-662, Richmond, VA 23298, USA.
Expert Rev Proteomics. 2004 Dec;1(4):485-92. doi: 10.1586/14789450.1.4.485.
Identifying the proteins and their complex interactions that promote and/or sustain the aggressive malignant phenotype is essential for understanding key effectors of the molecular biology of prostate cancer. This is also essential for development of new clinical applications. A variety of proteomic techniques, ranging from mass spectrometry to new methods of multiplexing protein identification, have great potential for rapidly achieving these goals. However, in order to obtain meaningful results, these techniques must be applied within the context of our knowledge of the heterogeneity of prostate tissues and tumors, the impact of specimen processing on both the quality and quantity of proteins detected and a thorough understanding of prostate cell biology. Collaboration between the protein chemist and the prostate cell biologist will expedite progress in this important field.
识别促进和/或维持侵袭性恶性表型的蛋白质及其复杂相互作用，对于理解前列腺癌分子生物学的关键效应器至关重要。这对于开发新的临床应用也至关重要。从质谱分析到蛋白质多重鉴定的新方法等各种蛋白质组学技术，在快速实现这些目标方面具有巨大潜力。然而，为了获得有意义的结果，这些技术必须在我们对前列腺组织和肿瘤异质性的了解、样本处理对检测到的蛋白质质量和数量的影响以及对前列腺细胞生物学的透彻理解的背景下应用。蛋白质化学家与前列腺细胞生物学家之间的合作将加快这一重要领域的进展。
