脑脊液蛋白质组：用于潜在生物标志物鉴定的蛋白质库。

CSF proteome: a protein repository for potential biomarker identification.

作者信息

Romeo Martin J, Espina Virginia, Lowenthal Mark, Espina Benjamin H, Petricoin Emanuel F, Liotta Lance A

机构信息

Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA.

出版信息

Expert Rev Proteomics. 2005 Jan;2(1):57-70. doi: 10.1586/14789450.2.1.57.

DOI:10.1586/14789450.2.1.57

PMID:15966853

Abstract

Proteomic analysis is not limited to the analysis of serum or tissues. Synovial, peritoneal, pericardial and cerebrospinal fluid represent unique proteomes for disease diagnosis and prognosis. In particular, cerebrospinal fluid serves as a rich source of putative biomarkers that are not solely limited to neurologic disorders. Peptides, proteolytic fragments and antibodies are capable of crossing the blood-brain barrier, thus providing a repository of pathologic information. Proteomic technologies such as immunoblotting, isoelectric focusing, 2D gel electrophoresis and mass spectrometry have proven useful for deciphering this unique proteome. Cerebrospinal fluid proteins are generally less abundant than their corresponding serum counterparts, necessitating the development and use of sensitive analytical techniques. This review highlights some of the promising areas of cerebrospinal fluid proteomic research and their clinical applications.

摘要

蛋白质组学分析并不局限于血清或组织分析。滑膜液、腹腔液、心包液和脑脊液代表了用于疾病诊断和预后的独特蛋白质组。特别是，脑脊液是潜在生物标志物的丰富来源，这些生物标志物不仅限于神经系统疾病。肽、蛋白水解片段和抗体能够穿过血脑屏障，从而提供病理信息库。免疫印迹、等电聚焦、二维凝胶电泳和质谱等蛋白质组学技术已被证明对解读这一独特蛋白质组很有用。脑脊液蛋白通常比其相应的血清蛋白含量少，因此需要开发和使用灵敏的分析技术。本综述重点介绍了脑脊液蛋白质组学研究的一些有前景的领域及其临床应用。