Universidad Nacional Autónoma de México, Instituto de Química, Ciudad Universitaria, 04510, Mexico City, Mexico.
Departamento de Química Analítica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 Cd., Mexico City, Mexico.
Metabolomics. 2021 Apr 16;17(5):40. doi: 10.1007/s11306-021-01794-3.
Brain death (BD) is the irreversible cessation of all functions of the entire brain, including the brainstem. Cerebrospinal fluid (CSF) is a biological liquid that circulates in brain and spine. Metabolomics is able to reveal the response of biological systems to diverse factors in a specific moment or condition. Therefore, the study of this neurological condition through metabolic profiling using high resolution Nuclear Magnetic Resonance (NMR) spectroscopy is important for understanding biochemical events.
The aim of the current study is to identify the metabolomics signature of BD using H-NMR spectroscopy in human CSF.
H-NMR spectroscopy has been employed for metabolomic untargeted analysis in 46 CSF samples: 22 control and 24 with BD. Spectral data were further subjected to multivariate analysis.
Statistically significant multivariate models separated subject's samples with BD from controls and revealed twenty one discriminatory metabolites. The statistical analysis of control and BD subjects using Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) model resulted in RX of 0.733 and Q of 0.635. An elevation in the concentration of statistically discriminant metabolites in BD was observed.
This study identifies a metabolic signature associated with BD and the most relevant enriched selected metabolic pathways.
脑死亡（BD）是整个大脑，包括脑干的所有功能不可逆转的停止。脑脊液（CSF）是一种在大脑和脊柱中循环的生物液体。代谢组学能够揭示生物系统对特定时刻或条件下多种因素的反应。因此，通过使用高分辨率核磁共振（NMR）光谱对代谢组学进行分析，研究这种神经系统疾病对于了解生化事件非常重要。
本研究旨在使用 H-NMR 光谱在人 CSF 中鉴定 BD 的代谢组学特征。
采用 H-NMR 光谱对 46 例 CSF 样本进行非靶向代谢组学分析：22 例对照和 24 例 BD。对光谱数据进行了多元分析。
统计上显著的多元模型将 BD 患者的样本与对照组区分开来，并揭示了二十一个有区别的代谢物。使用正交投影到潜在结构判别分析（OPLS-DA）模型对对照和 BD 受试者的统计分析得出 RX 为 0.733，Q 为 0.635。BD 中统计学上有区别的代谢物浓度升高。
本研究确定了与 BD 相关的代谢特征以及最相关的富集选择代谢途径。
