在一组巴西个体样本中，功能性白细胞介素-1β基因多态性与慢性牙周炎相关。
A functional interleukin-1 beta gene polymorphism is associated with chronic periodontitis in a sample of Brazilian individuals.
作者信息
Moreira Paula R, de Sá Alessandra R, Xavier Guilherme M, Costa José E, Gomez Ricardo S, Gollob Kenneth J, Dutra Walderez O
机构信息
Laboratory of Cell-Cell Interactions, Department of Morphology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
出版信息
J Periodontal Res. 2005 Aug;40(4):306-11. doi: 10.1111/j.1600-0765.2005.00801.x.
BACKGROUND
Interleukin-1 beta (IL-1 beta) is a potent inflammatory mediator and an important polymorphism in the locus +3954 (C/T) of the human IL1 B gene has been shown to affect the levels of this cytokine. This functional polymorphism has been associated with the establishment of inflammatory diseases, including periodontal disease, in European, Asian and North American populations.
OBJECTIVE
The aim of this study was to investigate the association between the IL1 B (+3954) gene polymorphism and the occurrence of different clinical forms of periodontitis in a sample of Brazilian individuals.
METHODS
This study employed a cross-sectional design involving individuals from the State of Minas Gerais in the south-eastern region of Brazil. Genomic DNA was obtained from oral swabs of 129 individuals and amplified using the polymerase chain reaction (PCR) with specific primers flanking the locus +3954 of IL1 B. PCR products were submitted to restriction endonuclease digestion and analyzed by polyacrylamide gel electrophoresis, to distinguish alleles T and C of the IL1 B gene, allowing for the determination of the genotypes and detection of the polymorphism.
RESULTS AND CONCLUSIONS
The chronic periodontitis group displayed a higher percentage of the T allele (28%) when compared to the aggressive periodontitis group (10.7%, chi(2)=5.24, p=0.02, OR=0.31, CI=0.11--0.88) and to control group (8.7%, chi(2)=7.11, p=0.007, OR=0.24, CI=0.08--0.73). Our data suggested that the polymorphism in the locus +3954 of IL1 B gene could be a risk factor for chronic periodontitis in a sample of Brazilian individuals.
背景
白细胞介素-1β（IL-1β）是一种强效炎症介质，人类IL1B基因+3954位点（C/T）的一种重要多态性已被证明会影响这种细胞因子的水平。在欧洲、亚洲和北美人群中，这种功能多态性与包括牙周病在内的炎症性疾病的发生有关。
目的
本研究旨在调查巴西人群样本中IL1B（+3954）基因多态性与不同临床类型牙周炎发生之间的关联。
方法
本研究采用横断面设计，研究对象来自巴西东南部米纳斯吉拉斯州的个体。从129名个体的口腔拭子中获取基因组DNA，并使用聚合酶链反应（PCR），以位于IL1B基因+3954位点两侧的特异性引物进行扩增。PCR产物进行限制性内切酶消化，并通过聚丙烯酰胺凝胶电泳进行分析，以区分IL1B基因的T和C等位基因，从而确定基因型并检测多态性。
结果与结论
与侵袭性牙周炎组（10.7%，χ²=5.24，p=0.02，OR=0.31，CI=0.11-0.88）和对照组（8.7%，χ²=7.11，p=0.007，OR=0.24，CI=0.08-0.73）相比，慢性牙周炎组的T等位基因百分比更高（28%）。我们的数据表明，IL1B基因+3954位点的多态性可能是巴西人群样本中慢性牙周炎的一个危险因素。
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