Health and Biosciences School, Pontifícia Universidade Católica do Paraná (PUCPR), Imaculada Conceição Street 1155, CEP 80215-901, Curitiba, PR, Brazil.
Cytokine. 2012 Oct;60(1):76-82. doi: 10.1016/j.cyto.2012.06.006. Epub 2012 Jul 13.
Chronic kidney disease (CKD) and periodontitis (PD) are complex inflammatory disturbances, influenced by genetic factors. Interleukin (IL)-1 genes code for inflammatory mediators involved in the physiopathogenesis of both diseases. Functional polymorphisms in IL1 genes modulate cytokine levels and have been associated with susceptibility to immune-inflammatory conditions.
The aim of this study was investigate the association of functional IL1 gene polymorphisms and transcript levels with susceptibility to CKD and PD.
The sample consisted of 246 individuals, mean age 44.8 years, divided into: group 1 (64 patients without CKD and without PD), group 2 (58 without CKD and with PD), group 3 (52 with CKD and without PD) and group 4 (72 with CKD and with PD). DNA was obtained from cells of oral mucosa and polymorphisms IL1AC-889T, IL1BC-511T, IL1BC+3954T and IL1RN (intron 2) were analyzed by PCR-RFLP. Transcript levels from gingival tissues were analyzed by real-time PCR.
IL1RN()1 allele was associated with almost 4-fold increased risk for CKD (OR 3.92 95% CI=1.6-9.4, p=0.002). IL1RN()2 allele was associated with 3-fold increased risk for PD in CKD patients (OR 3.08 95% CI=1.2-7.9, p=0.019). Allele T for polymorphism IL1B+3954 was associated with CKD in PD patients (OR 2.28 95% CI=1.1-4.7, p=0.019). Significantly increased levels of transcripts of IL1A, IL1B and IL1RN genes were found in PD patients.
It was observed an evidence for association of IL1B and IL1RN alleles with susceptibility to CKD and PD. Higher levels of IL1 gene transcripts were found in PD patients.
本研究旨在探讨白细胞介素（IL）1 基因多态性与转录水平与慢性肾脏病（CKD）和牙周炎（PD）易感性的关系。
该样本由 246 名年龄 44.8 岁的个体组成，分为 4 组：组 1（64 例无 CKD 且无 PD）、组 2（58 例无 CKD 但有 PD）、组 3（52 例有 CKD 但无 PD）和组 4（72 例有 CKD 且有 PD）。从口腔黏膜细胞中提取 DNA，采用 PCR-RFLP 分析白细胞介素 1AC-889T、白细胞介素 1BC-511T、白细胞介素 1BC+3954T 和白细胞介素 1RN（内含子 2）多态性。采用实时 PCR 分析牙龈组织中的转录水平。
白细胞介素 1RN()1 等位基因与 CKD 的风险增加近 4 倍（OR 3.92 95%CI=1.6-9.4，p=0.002）相关。白细胞介素 1RN()2 等位基因与 CKD 患者 PD 的风险增加 3 倍相关（OR 3.08 95%CI=1.2-7.9，p=0.019）。IL1B+3954 多态性的 T 等位基因与 PD 患者的 CKD 相关（OR 2.28 95%CI=1.1-4.7，p=0.019）。在 PD 患者中发现白细胞介素 1A、白细胞介素 1B 和白细胞介素 1RN 基因的转录水平显著升高。
观察到白细胞介素 1B 和白细胞介素 1RN 等位基因与 CKD 和 PD 的易感性相关。在 PD 患者中发现白细胞介素基因转录水平升高。
