Department of Periodontics, College of Dentistry, University of Tikrit, Tikrit, Iraq.
Department of Periodontics, College of Dentistry, University of Anbar, Anbar, Iraq.
Arch Razi Inst. 2023 Apr 30;78(2):751-756. doi: 10.22092/ARI.2022.359864.2491. eCollection 2023 Apr.
Periodontitis is a complex multifactorial inflammatory disease, and its genetic basis has been studied. The Interleukin-1 beta (IL-1β) is a crucial proinflammatory mediator in the pathogenesis of periodontitis with high polymorphism. This study aimed to investigate whether the rs1143634 genetic variant of the IL-1β gene is associated with an increased risk for periodontitis. For this purpose, genotyping of the IL-1β rs1143634 polymorphism was performed using the polymerase chain reaction-restriction fragment length polymorphism method on 90 patients within the age range of 35-60 years old. They were divided into two groups: 64 periodontitis cases (stage 3 and 4 periodontitis according to 2017 classification) and 26 racially matched healthy cases as the control group. Fisher's exact test showed a significant decrease in TT homozygous genotype in periodontitis cases, compared to the control group (=0.018), suggesting that this genotype is a protective factor in the test population. Allele frequency showed an elevated odd ratio (1.24) and increased risk for periodontitis in subjects with allele C and reduced odd ratio (0.81) and reduced risk for periodontitis in subjects with allele T. Allele T of IL-1β rs1143634 polymorphism could be a protective factor, while Allele C of this polymorphism could be a risk factor for periodontitis in the studied Iraqi population.
牙周炎是一种复杂的多因素炎症性疾病，其遗传基础已得到研究。白细胞介素-1β（IL-1β）是牙周炎发病机制中的关键促炎介质，具有高度多态性。本研究旨在探讨白细胞介素-1β基因的 rs1143634 遗传变异是否与牙周炎的风险增加有关。为此，采用聚合酶链反应-限制性片段长度多态性方法对 90 例年龄在 35-60 岁之间的患者进行了白细胞介素-1β rs1143634 多态性基因分型。他们被分为两组：64 例牙周炎病例（根据 2017 年分类为 3 期和 4 期牙周炎）和 26 例种族匹配的健康对照病例。Fisher 确切检验显示，与对照组相比，牙周炎病例的 TT 纯合基因型显著减少（=0.018），表明该基因型在试验人群中是一种保护因素。等位基因频率显示，等位基因 C 增加了患牙周炎的风险（比值比 1.24），而等位基因 T 降低了患牙周炎的风险（比值比 0.81）。白细胞介素-1β rs1143634 多态性的等位基因 T 可能是一种保护因素，而该多态性的等位基因 C 可能是研究中伊拉克人群患牙周炎的风险因素。
