Aki C, Yilmaz S, Dilgin Y, Yagmur S, Suren E
Department of Molecular Biology, Faculty of Arts and Sciences, University of Canakkale Onsekiz Mart, Canakkale, Turkey.
Pharmazie. 2005 Oct;60(10):747-50.
The electrochemical oxidation and determination of natamycin has been carried out at a carbon paste electrode in aqueous solutions in the pH range of 2.5-10.30 by cyclic and differential pulse voltammetry. Best results were obtained with the differential pulse voltammetric technique in 0.5 M sulfuric acid at pH 1.82. The diffusion controlled nature of the waves was established. A differential pulse voltammetric technique for the determination of natamycin 0.5 M sulfuric acid which allows quantitation over the range of 2 x 10(-6)-8 x 10(-5) M range method is proposed. Limit of detection and limit of quantitatification were 1.5 x 10(-6) and 5 x 10(-6) M, respectively. Based on this study a simple, rapid, selective and sensitive voltammetric method was developed for the determination of natamycin in capsules. In order to validate the proposed method, UV spectroscopy was applied.
已通过循环伏安法和差分脉冲伏安法在碳糊电极上于pH值为2.5 - 10.30的水溶液中对纳他霉素进行了电化学氧化及测定。在pH值为1.82的0.5 M硫酸中采用差分脉冲伏安技术获得了最佳结果。确定了波的扩散控制性质。提出了一种在0.5 M硫酸中测定纳他霉素的差分脉冲伏安技术，该方法可在2×10⁻⁶ - 8×10⁻⁵ M范围内进行定量。检测限和定量限分别为1.5×10⁻⁶ M和5×10⁻⁶ M。基于此研究，开发了一种简单、快速、选择性好且灵敏的伏安法用于测定胶囊中的纳他霉素。为验证所提出的方法，应用了紫外光谱法。
