Casado-Terrones S, Fernández-Sánchez J F, Díaz B Cañabate, Carretero A Segura, Fernández-Gutiérrez A
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, C/Fuentenueva s/n, 18071 Granada, Spain.
J Pharm Biomed Anal. 2005 Jul 15;38(4):785-9. doi: 10.1016/j.jpba.2005.02.018. Epub 2005 Mar 23.
We have developed an optical sensor for determining and quantifying naphazoline (NPZ) based on its inherent fluorescence property. We have placed a non-ionic-exchanger solid support (Amberlite XAD-7) in a flow cell in the light path of the excitation beam and the fluorescence signal for NPZ is continuously monitored at lambda(exc/nm)=294/306 nm. The response time for this sensor is acceptably fast, 80s, obtaining a detection limit of 2.6 ng mL(-1) with standard deviations of 2.0% at 125 ng mL(-1). This device has been satisfactorily applied to two commercial formulations and its selectivity has been demonstrated with an interference study. The advantages have been compared with the only published sensor for determining NPZ in pharmaceutical preparations and with other analytical methods in the literature.
我们基于萘甲唑啉（NPZ）的固有荧光特性开发了一种用于测定和定量萘甲唑啉的光学传感器。我们将非离子交换固体支持物（Amberlite XAD - 7）置于激发光束光路中的流通池中，并在λ（exc/nm）= 294/306 nm处连续监测NPZ的荧光信号。该传感器的响应时间较快，为80秒，在125 ng mL⁻¹时检测限为2.6 ng mL⁻¹，标准偏差为2.0%。该装置已成功应用于两种商业制剂，并通过干扰研究证明了其选择性。已将其优势与文献中唯一已发表的用于测定药物制剂中NPZ的传感器以及其他分析方法进行了比较。
