Pescheck M, Schrader J, Sell D
Biochemical Engineering Group, Karl-Winnacker-Institut der DECHEMA e.V., Theodor-Heuss-Allee 25, 60486 Frankfurt am Main, Germany.
Bioelectrochemistry. 2005 Sep;67(1):47-55. doi: 10.1016/j.bioelechem.2004.10.004. Epub 2005 Feb 25.
A novel amperometric sensor system is presented which directly reflects the metabolic activity of prokaryotic and eukaryotic cells during cultivation. The principle of an externally mounted sensor is current measurement using a three-electrode system. Only living cells are detected since the current signal is based on a redox mediator. Added to a culture sample in its oxidized form, the mediator is reduced by cellular metabolism and subsequently re-oxidized at the anode. The spontaneous immobilisation of the cells in the reaction vessel of the sensor by swelling dextrane polymers (Sephadex) prior to measurement is the key to a fast, consistent signal. Even metabolically less active mammalian cells produce a reliable signal within a few minutes; this may open up future applications of the electrochemical sensor in closed loop process control not only for bacterial and fungal bioprocesses, but also in cell culture technology.
本文介绍了一种新型安培传感器系统，该系统可直接反映原核细胞和真核细胞在培养过程中的代谢活性。外部安装传感器的原理是使用三电极系统进行电流测量。由于电流信号基于氧化还原介质，因此仅能检测到活细胞。以氧化形式添加到培养样品中的介质，会被细胞代谢还原，随后在阳极重新氧化。在测量前通过溶胀葡聚糖聚合物（葡聚糖凝胶）使细胞自发固定在传感器的反应容器中，这是获得快速、稳定信号的关键。即使是代谢活性较低的哺乳动物细胞也能在几分钟内产生可靠的信号；这可能为电化学传感器在闭环过程控制中的未来应用开辟道路，不仅适用于细菌和真菌生物过程，也适用于细胞培养技术。
