Sandeman Susan R, Howell Carol A, Phillips Gary J, Lloyd Andrew W, Davies J Graham, Mikhalovsky Sergey V, Tennison Steve R, Rawlinson Andrew P, Kozynchenko Oleksandr P, Owen Hannah L H, Gaylor John D S, Rouse Jennifer J, Courtney James M
Biomedical Materials Research Group, School of Pharmacy and Biomolecular Sciences, University of Brighton, UK.
Biomaterials. 2005 Dec;26(34):7124-31. doi: 10.1016/j.biomaterials.2005.05.019.
The aim of the present study was to conduct a preliminary investigation into the blood biocompatibility of a novel, uncoated carbon for use in a filtration/adsorption device for the treatment of sepsis. Carbon well prototypes were manufactured from phenol-formaldehyde-aniline-based pyrolysed carbons using monolithic polymer technology. Inflammatory blood cell and plasma protein mediation of the inflammatory response were evaluated using the novel carbon prototypes and compared with dialyser membrane and tissue culture plate controls. Assays determining monocyte and granulocyte adhesion, platelet adhesion and activation, granulocyte activation and complement activation were performed. Preliminary findings suggest an adsorptive but passivating carbon surface. Moderate levels of monocyte and granulocytes adhesion were seen in conjunction with adsorption of plasma proteins to the carbon surface. Activation of granulocyte and adherent platelets was not detected and the complement cascade was not activated by the carbons, indicating a surface compatible with blood contact. The results support the further development of the proposed carbon-based device for the treatment of sepsis.
本研究的目的是对一种新型未涂层碳的血液生物相容性进行初步研究，该碳用于治疗败血症的过滤/吸附装置。碳阱原型采用整体聚合物技术，由基于苯酚-甲醛-苯胺的热解碳制成。使用新型碳原型评估炎症血细胞和血浆蛋白对炎症反应的介导作用，并与透析器膜和组织培养板对照进行比较。进行了测定单核细胞和粒细胞粘附、血小板粘附和活化、粒细胞活化和补体活化的试验。初步研究结果表明，碳表面具有吸附性但呈钝化状态。在血浆蛋白吸附到碳表面的同时，观察到中等水平的单核细胞和粒细胞粘附。未检测到粒细胞和粘附血小板的活化，碳也未激活补体级联反应，表明该表面与血液接触相容。这些结果支持进一步开发所提议的用于治疗败血症的碳基装置。
