Shih Mei-Fen, Shau Min-Da, Chang Meng-Ying, Chiou Se-Kai, Chang Jiunn-Kae, Cherng Jong-Yuh
Chia Nan University of Pharmacy and Science, 60 Erh-Jen Rd., Sec. 1, Jen-Te, Tainan 717, Taiwan, ROC.
Int J Pharm. 2006 Dec 11;327(1-2):117-25. doi: 10.1016/j.ijpharm.2006.07.043. Epub 2006 Jul 29.
The aim of this study is to investigate how the presence of liquid crystal, cholesteryl oleyl carbonate, embedded into polymers (PMMA, Eb270, PU) affects the biocompatibility of composite membranes with human blood. The effects of different surface textures of composite membranes on platelet adhesion and platelet activation were evaluated as well. The adhesion and geometric deformation of platelets were demonstrated by SEM. The quantitative assay of platelet activation was determined by measuring the production of P-Selectin, and by measurement of the blood clotting index when PRP blood was incubated with pure polymer films and composite membranes. Moreover, the hemolysis studies on the damage to red blood cells were performed to gain information on the hemocompatibility of these biomaterials. The results showed that inclusion of cholesteryl oleyl carbonate (COC) embedded in composite membranes, improves their biocompatibility with respect to a substantial reduction of platelet adhesion and the controlled decrease of platelet activation. As the COC content of composite membranes was increased, the value of the blood clotting index increased and the production of P-Selectin decreased. The results also showed that the presence of COC resulted in a decrease of hemolysis ratios. Comparing among three different composite membranes, the best biocompatibility is achieved when PU/COC> or ==Eb270/COC>PMMA/COC. The in vitro studies performed in this work suggest that it may be reasonable to use liquid crystal COC as a mean of surface modification to improve the blood compatibility of biopolymers.
本研究的目的是探究嵌入聚合物（聚甲基丙烯酸甲酯、Eb270、聚氨酯）中的液晶胆甾醇油酸酯的存在如何影响复合膜与人血的生物相容性。还评估了复合膜不同表面纹理对血小板粘附和血小板活化的影响。通过扫描电子显微镜展示血小板的粘附和几何变形。通过测量P-选择素的产生以及当富血小板血浆与纯聚合物膜和复合膜孵育时测量凝血指数来确定血小板活化的定量测定。此外，进行了对红细胞损伤的溶血研究，以获取有关这些生物材料血液相容性的信息。结果表明，复合膜中嵌入胆甾醇油酸酯（COC）可改善其生物相容性，这表现为血小板粘附大幅减少以及血小板活化得到控制的降低。随着复合膜中COC含量的增加，凝血指数值增加而P-选择素的产生减少。结果还表明，COC的存在导致溶血率降低。在三种不同的复合膜之间进行比较，当聚氨酯/COC>或==Eb270/COC>聚甲基丙烯酸甲酯/COC时，可实现最佳生物相容性。本工作中进行的体外研究表明，使用液晶COC作为表面改性手段以改善生物聚合物的血液相容性可能是合理的。
