Du Jing, Liu Ge-Fei, Wang Gui-Ling, Xu Xiao-Lin, Wang Bo, Zhu Li
Department of Biochemistry, Shenyang Medical College, Shenyang, 110034, China.
Sheng Wu Gong Cheng Xue Bao. 2004 Jul;20(4):520-5.
This study describes the cDNA sequencing and the bioinformatic analysis of a novel NADP(H)-dependent retinol dehydrogenase/reductases isoform (NRDRiso). Based upon the concensus sequences of human and mouse NRDR coding region, we have identified a short 377 bp RT-PCR product from human liver tissue. The cDNA sequence of a NRDR isoform was then isolated using RACE approach and its sequence was analysed. The full-length cDNA is 1,003bp in length and was submitted to GenBank as NADP-dependent retinol dehydrogenase/reductase short isoform (NRDRiso). The open reading frames of NRDRiso cDNA is 525 bp.
本研究描述了一种新型烟酰胺腺嘌呤二核苷酸磷酸（NADP(H)）依赖性视黄醇脱氢酶/还原酶同工型（NRDRiso）的cDNA测序及生物信息学分析。基于人和小鼠NRDR编码区的共有序列，我们从人肝组织中鉴定出一段377 bp的短RT-PCR产物。然后采用RACE方法分离出NRDR同工型的cDNA序列并进行分析。全长cDNA长度为1,003 bp，作为烟酰胺腺嘌呤二核苷酸磷酸依赖性视黄醇脱氢酶/还原酶短同工型（NRDRiso）提交至GenBank。NRDRiso cDNA的开放阅读框为525 bp。
