Zhang Aijun, Hartung John S
U.S. Department of Agriculture, Agricultural Research Service, Chemicals Affecting Insect Behavior Laboratory, and Fruit Laboratory, Beltsville Agriculture Research Center-West, Maryland 20705, USA.
J Agric Food Chem. 2005 Jun 29;53(13):5134-7. doi: 10.1021/jf050533x.
An aldehyde oxime O-methyl ether, phenylacetaldehyde O-methyloxime, was detected using solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) in the headspace above grapefruit leaves infected with Xanthomonas axonopodis pv. citri, the causal agent of citrus bacterial canker disease (CBCD). This disease is a major phytosanitary concern, and an eradication campaign against it is currently underway in Florida. Phenylacetaldehyde O-methyloxime has been reported to be produced by other plants and fragrant flowers, but it was not observed in the headspace above uninfected grapefruit leaves, the pathogenic bacterium X. axonopodis pv. citri itself, or grapefruit leaves infected with another closely related bacterial pathogen, X. axonopodis pv. citrumelo, which causes citrus bacterial spot, a disease of no phytosanitary significance. It was also not detected from CBCD infected fruits, including orange, lemon, grapefruit, and lime. We conclude that phenylacetaldehyde O-methyloxime may potentially be used to identify CBCD infestations. However, more intensive studies will be required to fully evaluate the potential of phenylacetaldehyde O-methyloxime as a diagnostic compound for CBCD. Using SPME and GC-MS to measure phenylacetaldehyde O-methyloxime may provide an easy and feasible tool to complement current methods used to detect X. axonopodis pv. citri in environmental samples.
使用固相微萃取（SPME）和气相色谱-质谱联用（GC-MS）技术，在感染了柑橘溃疡病菌（柑橘细菌性溃疡病的病原体）的葡萄柚叶片上方的顶空中检测到了一种醛肟O-甲基醚，即苯乙醛O-甲基肟。这种疾病是一个主要的植物检疫问题，目前佛罗里达州正在开展针对它的根除行动。据报道，苯乙醛O-甲基肟由其他植物和香花产生，但在未感染的葡萄柚叶片、柑橘溃疡病菌本身或感染了另一种密切相关细菌病原体——引起柑橘细菌性斑点病（一种无植物检疫意义的疾病）的柑橘轴状叶点霉的葡萄柚叶片上方的顶空中未观察到。在感染柑橘细菌性溃疡病的果实（包括橙子、柠檬、葡萄柚和酸橙）中也未检测到。我们得出结论，苯乙醛O-甲基肟可能有潜力用于识别柑橘细菌性溃疡病的侵染。然而，需要更深入的研究来全面评估苯乙醛O-甲基肟作为柑橘细菌性溃疡病诊断化合物的潜力。使用SPME和GC-MS测量苯乙醛O-甲基肟可能提供一种简单可行的工具，以补充目前用于检测环境样品中柑橘溃疡病菌的方法。
